Anti-NT5C2 antibody (ab96084)
Overview
- Product nameAnti-NT5C2 antibodySee all NT5C2 primary antibodies ...
- DescriptionRabbit polyclonal to NT5C2
- Tested applicationsWB, IHC-P more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Zebrafish - Immunogen
Recombinant fragment, containing a sequence corresponding to a region within amino acids 108 and 332 of Human NT5C2.
- Positive controlH1299 and HeLaS3 whole cell lysate, OVCA tissue
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab96084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/3000. Predicted molecular weight: 65 kDa. |
| IHC-P | IHC-P: 1/100 - 1/250. |
Target
- FunctionMay have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.
- Sequence similaritiesBelongs to the 5'(3')-deoxyribonucleotidase family.
- Cellular localizationCytoplasm.
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Database links
- Entrez Gene: 281951 Cow
- Entrez Gene: 22978 Human
- Entrez Gene: 76952 Mouse
- Entrez Gene: 324845 Zebrafish
- Omim: 600417 Human
- SwissProt: O46411 Cow
- SwissProt: P49902 Human
- SwissProt: Q3V1L4 Mouse
- Unigene: 97439 Human
- Unigene: 40965 Mouse
see all
Target information above from: UniProt accession
P49902
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 5' nucleotidase (purine) cytosolic type B antibody5NTC_HUMAN antibodycN II antibody
- Cytosolic 5''-nucleotidase II antibodyCytosolic IMP/GMP-specific 5'-nucleotidase antibodyCytosolic purine 5' nucleotidase antibodyCytosolic purine 5''-nucleotidase antibodyGMP antibodyNT5B antibodynt5c2 antibodyNT5CP antibodyNUCLEOTIDASE, 5-PRIME, CYTOSOLIC II antibodyNUCLEOTIDASE, 5-PRIME, TYPE B antibodyPNT5 antibodyPURINE 5-PRIME-NUCLEOTIDASE antibody
see all
Anti-NT5C2 antibody images
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Western blot - NT5C2 antibody (ab96084)All lanes : Anti-NT5C2 antibody (ab96084) at 1/1000 dilution
Lane 1 : H1299 cell lysate
Lane 2 : HeLaS3 cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size : 65 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NT5C2 antibody (ab96084)Immunohistochemical analysis of paraffin-embedded OVCA, using ab96084 at 1:100 dilution.
References for Anti-NT5C2 antibody (ab96084)
This product has been referenced in:
- Tzoneva G et al. Activating mutations in the NT5C2 nucleotidase gene drive chemotherapy resistance in relapsed ALL. Nat Med 19:368-71 (2013). WB . Read more (PubMed: 23377281) »
