Overview

  • Product name
    Anti-NT5C2 antibody
  • Description
    Rabbit polyclonal to NT5C2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Zebrafish
  • Immunogen

    Recombinant fragment, containing a sequence corresponding to a region within amino acids 108 and 332 of Human NT5C2.

  • Positive control
    • H1299 and HeLaS3 whole cell lysate, OVCA tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 65 kDa.
IHC-P 1/100 - 1/250.

Target

  • Function
    May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.
  • Sequence similarities
    Belongs to the 5'(3')-deoxyribonucleotidase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5' nucleotidase (purine) cytosolic type B antibody
    • 5NTC_HUMAN antibody
    • cN II antibody
    • Cytosolic 5''-nucleotidase II antibody
    • Cytosolic IMP/GMP-specific 5'-nucleotidase antibody
    • Cytosolic purine 5' nucleotidase antibody
    • Cytosolic purine 5''-nucleotidase antibody
    • GMP antibody
    • NT5B antibody
    • nt5c2 antibody
    • NT5CP antibody
    • NUCLEOTIDASE, 5-PRIME, CYTOSOLIC II antibody
    • NUCLEOTIDASE, 5-PRIME, TYPE B antibody
    • PNT5 antibody
    • PURINE 5-PRIME-NUCLEOTIDASE antibody
    see all

Images

  • All lanes : Anti-NT5C2 antibody (ab96084) at 1/1000 dilution

    Lane 1 : H1299 cell lysate
    Lane 2 : HeLaS3 cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 65 kDa
  • Immunohistochemical analysis of paraffin-embedded OVCA, using ab96084 at 1:100 dilution.

References

This product has been referenced in:
  • Tzoneva G  et al. Activating mutations in the NT5C2 nucleotidase gene drive chemotherapy resistance in relapsed ALL. Nat Med 19:368-71 (2013). WB . Read more (PubMed: 23377281) »
  • Meyer JA  et al. Relapse-specific mutations in NT5C2 in childhood acute lymphoblastic leukemia. Nat Genet 45:290-4 (2013). Read more (PubMed: 23377183) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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