NTAL antibody [NAP-07] (ab3992)

Product Name 

NTAL antibody [NAP-07]

Product type 

Primary antibodies

Description 

Mouse monoclonal [NAP-07] to NTAL

Immunogen 

Recombinant fragment, corresponding to amino acids 91-244 of Human NTAL.

Reacts with 
(species key)

Hu, Ms

Specificity 

The antibody reacts with Non-T cell activation linker (NTAL) a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.

Tested applications 
(see key)

ICC/IF, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent concentration.
WB: Use at a concentration of 2 µg/ml. Use under non reducing conditions. Use Lysis buffer with 1% laurylmaltoside. (see Tkaczyk et al).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

RAMOS human lymphoma cell lysate, Raji human lymphoma cell lysate

Cellular localization 

Transmembrane protein

Research areas 

Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Other
Immunology >> Adaptive Immunity >> T Cells >> Non-CD
Immunology >> Innate Immunity >> Macrophage / Inflamm.

Relevance 

NTAL is involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2. Defects in NTAL may be a cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS). WBS is a rare developmental disorder

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

NAP-07

Isotype 

IgG1

Purity 

>95% by SDS-PAGE

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from hybridoma culture supernatant by protein A affinity chromatography.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for NTAL antibody [NAP-07] images, references, products related to ab3992 and other tools.

NTAL antibody [NAP-07] images:

References for NTAL antibody [NAP-07] (ab3992)

  This product has been used in: (two most recent references)

Malhotra S et al. Vav and Rac activation in B cell antigen receptor endocytosis involves Vav recruitment to the adapter protein LAB. J Biol Chem 284:36202-12 (2009). WB; Mouse. PubMed: 19858206

Mutch CM et al. Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes: evidence for a role in internalization of the B cell receptor. Int Immunol 19:19-30 (2007). PubMed: 17090619

See all 9 publication references for this product.


If you publish research using ab3992 please let us know so that we can cite the reference on this datasheet.

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