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Anti-NTAL antibody [NAP-07] (ab3992)

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Overview

Product name

Anti-NTAL antibody [NAP-07]
See all NTAL products (7) ...

Description

Mouse monoclonal [NAP-07] to NTAL

Specificity

The antibody reacts with Non-T cell activation linker (NTAL) a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.

Tested applications

WB, IP, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Recombinant fragment, corresponding to amino acids 91-244 of Human NTAL.

Positive control

RAMOS human lymphoma cell lysate, Raji human lymphoma cell lysate

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

>95% by SDS-PAGE

Purification notes

Purified from hybridoma culture supernatant by protein A affinity chromatography.

Clonality

Monoclonal

Clone number

NAP-07

Isotype

IgG1

Research areas

Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Other
Immunology >> Adaptive Immunity >> T Cells >> Non-CD
Immunology >> Innate Immunity >> Macrophage / Inflamm.

  • Immunocytochemistry/ Immunofluorescence - NTAL antibody [NAP-07] (ab3992)Immunocytochemistry/ Immunofluorescence - NTAL antibody [NAP-07] (ab3992) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2.

Tissue specificity

Highly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B-cells, NK cells and monocytes. Absent from T-cells (at protein level).

Involvement in disease

Note=LAT2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of LAT2 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.

Post-translational
modifications

Phosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2.
May be polyubiquitinated.

Cellular localization

Cell membrane. Present in lipid rafts.

Target information above from: UniProt accessionQ9GZY6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • HSPC046 antibody
  • LAB antibody
  • Lat2 antibody
  • Linker for activation of B cells antibody
  • Linker for activation of B-cells antibody
  • Linker for activation of T cells family member 2 antibody
  • Linker for activation of T cells transmembrane adaptor 2 antibody
  • Linker for activation of T-cells family member 2 antibody
  • Linker of Activated B cells antibody
  • Membrane associated adapter molecule antibody
  • Membrane-associated adapter molecule antibody
  • Non T Cell Activation Linker antibody
  • Non-T-cell activation linker antibody
  • NTAL_HUMAN antibody
  • WBSCR 5 antibody
  • Wbscr15 antibody
  • Williams Beuren syndrome chromosome region 15 antibody
  • Williams Beuren Syndrome chromosome region 5 antibody
  • Williams-Beuren syndrome chromosomal region 15 protein antibody
  • Williams-Beuren syndrome chromosomal region 5 protein antibody
  • WSCR5 antibody
see all

Database links

Anti-NTAL antibody [NAP-07] images:

  Immunocytochemistry/ Immunofluorescence - NTAL antibody [NAP-07] (ab3992)

Immunocytochemistry/ Immunofluorescence - NTAL antibody [NAP-07] (ab3992)

Subcellular localization of NTAL by confocal microscopy in the myeloid cell line THP-1. THP-1 cells were permeabilized and immunostained using antibody NAP-07 (green). Nuclei are vizualized by propidium iodide (red).

References for Anti-NTAL antibody [NAP-07] (ab3992)

This product has been referenced in:

  • Malhotra Set al. Vav and Rac activation in B cell antigen receptor endocytosis involves Vav recruitment to the adapter protein LAB. J Biol Chem 284:36202-12 (2009). WB; Mouse.Read more (PubMed: 19858206) »
  • Mutch CMet al. Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes: evidence for a role in internalization of the B cell receptor. Int Immunol 19:19-30 (2007).Read more (PubMed: 17090619) »

See all 8 publications for this product

Publishing research using ab3992? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"