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Anti-NTAL antibody (ab36452)

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Overview

Product name

Anti-NTAL antibody
See all NTAL products (7) ...

Description

Rabbit polyclonal to NTAL

Specificity

We have data to indicate that this antibody may not cross react with Human. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse

Immunogen

Recombinant fragment, corresponding to amino acids 30-229 of Mouse NTAL

Positive control

Mouse spleen.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein A purified

Purification notes

Purified from rabbit serum.

Primary antibody notes

This antibody reacts with mouse NTAL (25 kDa) but not human.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Other
Immunology >> Adaptive Immunity >> T Cells >> Non-CD
Immunology >> Innate Immunity >> Macrophage / Inflamm.

  • Western blot - NTAL antibody (ab36452)Western blot - NTAL antibody (ab36452) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab36452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 µg/ml. Use under non reducing condition. Detects a band of approximately 25 kDa.

Target

Function

Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2.

Tissue specificity

Highly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B-cells, NK cells and monocytes. Absent from T-cells (at protein level).

Involvement in disease

Note=LAT2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of LAT2 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.

Post-translational
modifications

Phosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2.
May be polyubiquitinated.

Cellular localization

Cell membrane. Present in lipid rafts.

Target information above from: UniProt accessionQ9GZY6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • HSPC046 antibody
  • LAB antibody
  • LAT2 antibody
  • Linker for activation of B cells antibody
  • Linker for activation of B-cells antibody
  • Linker for activation of T cells family member 2 antibody
  • Linker for activation of T cells transmembrane adaptor 2 antibody
  • Linker for activation of T-cells family member 2 antibody
  • Membrane associated adapter molecule antibody
  • Membrane-associated adapter molecule antibody
  • Non T cell activation linker antibody
  • Non-T-cell activation linker antibody
  • NTAL_HUMAN antibody
  • WBSCR 5 antibody
  • Wbscr15 antibody
  • Williams Beuren syndrome chromosome region 15 antibody
  • Williams Beuren syndrome chromosome region 5 antibody
  • Williams-Beuren syndrome chromosomal region 15 protein antibody
  • Williams-Beuren syndrome chromosomal region 5 protein antibody
  • WSCR5 antibody
see all

Database links

Anti-NTAL antibody images:

  Western blot - NTAL antibody (ab36452)

Western blot - NTAL antibody (ab36452)

All lanes : Anti-NTAL antibody (ab36452) at 1 µg/ml

Lane 1 : Mouse spleen tissue immunostained with rabbit polyclonal antibody to mouse Non-T cell Activation Linker (ab36452)
Lane 2 : Mouse spleen tissue immunostained with rabbit polyclonal antibody to mouse Non-T cell Activation Linker, N-terminal.
Lane 3 : Negative control


Performed under non-reducing conditions.

Observed band size : 25 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa (possible non-specific binding),75 kDa (possible non-specific binding),80 kDa (possible non-specific binding).

References for Anti-NTAL antibody (ab36452)

ab36452 has not yet been referenced specifically in any publications.

Publishing research using ab36452? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"