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Overview

  • Product nameNTF97/Importin beta proteinSee all NTF97/Importin beta proteins and peptides ...
  • Protein descriptionFull length Human NTF97/Importin beta protein (amino acids 1-876) with an N terminal His tag, predicted Mwt: 99.6 kDa inclusive of tag (NP_002256).
  • Uniprot accessionQ14974
  • Molecular weight99.600kDa inclusive of tags
  • Protein length876 amino acids
  • Expression hostE. coli

    • Properties

  • Purity> 85 % by SDS-PAGE
  • Purification notesab116172 is purified using conventional chromatography techniques.
  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 8.00
    Constituents: 30% Glycerol, 0.32% Tris HCl, 0.02% DTT, 0.58% Sodium chloride
  • Concentration information loading...
  • Sequence notesMGSSHHHHHHSSGLVPRGSHMGSQRWLAIDANARREVKNY VLQTLGTETYRPSSASQCVAGIACAEIPVNQWPELIPQLV ANVTNPNSTEHMKESTLEAIGYICQDIDPEQLQDKSNEIL TAIMELITILEKTVSPDRLE LEAAQKFLERAAVENLPTFLVELSRVLANPGNSQVARVAA GLQIKNSLTSKDPDIKAQYQIQGMRKEEPSNNVKLAATNA LLNSLEFTKANFDKESERHFIMQVVCEATQCPDTRVRVAA LQNLVKIMSLYYQYMETYMGPALFAITIEAMKSDIDEVAL QGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKG ALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLAT CCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGCILEGP EPSQLKPLVIQAMPTLIELMKDPSVVVRDTAAWTVGRICE LLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVCWAFSSL AEAAYEAADVADDQEEPATYCLSSSFELIVQKLLETTDRP DGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMER LQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQH QDALQISDVVMASLLRMFQSTAGSGGVQEDALMAVSTLVE VLGGEFLKYMEAFKPFLGIGLKNYAEYQVCLAAVGLVGDL CRALQSNIIPFCDEVMQLLLENLGNENVHRSVKPQILSVF GDIALAIGGEFKKYLEVVLNTLQQASQAQVDKSDYDMVDY LNELRESCLEAYTGIVQGLKGDQENVHPDVMLVQPRVEFI LSFIDHIAGDEDHTDGVVACAAGLIGDLCTAFGKDVLKLV EARPMIHELLTEGRRSKTNKAKTLATWATKELRKLKNQA
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab116172 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab45901,ab50417 and ab115882.

    • Protein info

    • Alternative names
        IMB 1IMB1IMB1_HUMAN
        ImpnbImportin 90Importin beta 1Importin beta 1 subunitImportin subunit beta-1Importin-90IPOBKaryopherin beta 1Karyopherin beta 1 subunitKaryopherin subunit beta-1KPNB 1Kpnb1MGC2155MGC2156MGC2157NTF 97NTF97Nuclear factor P97Pore targeting complex 97 kDa subunitPTAC97
      see all
  • FunctionFunctions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus.
  • Sequence similaritiesBelongs to the importin beta family.
    Contains 8 HEAT repeats.
    Contains 1 importin N-terminal domain.
  • Cellular localizationCytoplasm. Nucleus envelope.

    • Target information above from: UniProt accession Q14974 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    NTF97/Importin beta protein images

    • SDS-PAGE - NTF97/Importin beta protein (ab116172)
      SDS-PAGE - NTF97/Importin beta protein (ab116172)
      15% SDS-PAGE showing ab116172 at approximately 99.6kDa (3µg).

    References for NTF97/Importin beta protein (ab116172)

    ab116172 has not yet been referenced specifically in any publications.

    Publishing research using ab116172 ? Please let us know so that we can cite the reference in this datasheet

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