Anti-NUDT12 antibody (ab197310)
Key features and details
- Rabbit polyclonal to NUDT12
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NUDT12 antibody -
Description
Rabbit polyclonal to NUDT12 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Orangutan -
Immunogen
Recombinant fragment corresponding to Human NUDT12 aa 150-400. (BC041099).
Database link: Q9BQG2 -
Positive control
- HeLa, 293T, A549 and HepG2 cell lysates; Human fetal stomach tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute in 200 ul sterile H2O. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/200 - 1/1000. Predicted molecular weight: 52 kDa.
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IHC-P |
1/100 - 1/500.
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Notes |
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WB
1/200 - 1/1000. Predicted molecular weight: 52 kDa. |
IHC-P
1/100 - 1/500. |
Target
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Relevance
Nucleotides are involved in numerous biochemical reactions and pathways within the cell as substrates, cofactors, and effectors. Nudix hydrolases, such as NUDT12 (Nudix (nucleoside diphosphate linked moiety X)-type motif 12), regulate the concentrations of individual nucleotides and of nucleotide ratios in response to changing circumstances. NUDT12 hydrolyzes NAD(P)H to NMNH and AMP (2',5'-ADP), and diadenosine diphosphate to AMP. It also has moderate activity towards NAD(P)(+), ADP-ribose and diadenosine triphosphate. It may regulate the concentration of peroxisomal nicotinamide nucleotide cofactors required for oxidative metabolism. -
Cellular localization
Peroxisome -
Database links
- Entrez Gene: 83594 Human
- Entrez Gene: 67993 Mouse
- Omim: 609232 Human
- SwissProt: Q9BQG2 Human
- SwissProt: Q9DCN1 Mouse
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Alternative names
- 0610016O18Rik antibody
- DKFZP761I172 antibody
- EC 3.6.1.22 antibody
see all
Images
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All lanes : Anti-NUDT12 antibody (ab197310) at 1/500 dilution
Lane 1 : 293T cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
Predicted band size: 52 kDa -
Immunohistochemical analysis of formaliln-fixed, paraffin-embedded Human fetal stomach tissue labeling NUDT12 with ab197310 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab197310 has been referenced in 1 publication.
- Grudzien-Nogalska E et al. Structural and mechanistic basis of mammalian Nudt12 RNA deNADding. Nat Chem Biol 15:575-582 (2019). PubMed: 31101919