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Anti-NUMB antibody (ab4147)

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Overview

Product name

Anti-NUMB antibody
See all NUMB products (4) ...

Description

Goat polyclonal to NUMB

Specificity

This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.

Tested applications

IHC-P, WB, IP, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Human

Predicted to work with

Cow, Dog

Immunogen

Synthetic peptide: PFSSDLQKTFEIEL, corresponding to C terminal amino acids 638-651 of Human NUMB.

C-PFSSDLQK TFEIEL

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

A431 lysate. Mouse brain lysates.

General notes

This product may cross-react with Numblike (NUMBL, GeneID: 9253) Principal Names - NUMB; S171; numb homolog (Drosophila) Official Gene Symbol - NUMB GenBank Accession Number – NP_003735 LocusLink ID - 8650 (human); 18222 (mouse) Gene Ontology terms - integral to plasma membrane

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

Research areas

Stem Cells >> Signaling Pathways >> Notch >> Cytoplasmic
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Notch Pathway

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NUMB antibody (ab4147)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NUMB antibody (ab4147) image (enlarge)

  • Western blot - NUMB antibody (ab4147)Western blot - NUMB antibody (ab4147) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab4147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.

Sequence similarities

Contains 1 PID domain.

Post-translational
modifications

Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.

Cellular localization

Membrane.

Target information above from: UniProt accessionP49757 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • h Numb antibody
  • h-Numb antibody
  • Numb antibody
  • Numb homolog antibody
  • Numb protein homolog antibody
  • NUMB_HUMAN antibody
  • Protein numb homolog antibody
  • Protein S171 antibody
  • S171 antibody
see all

Database links

see all

Anti-NUMB antibody images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NUMB antibody (ab4147)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NUMB antibody (ab4147)

Single collagenase-isolated mouse muscle fibre stained with DAPI (blue) to reveal nuclei and anti-Numb (green) which detects satellite cells.

This picture was kindly supplied as part of the review submitted by Christopher Mann.

  Western blot - NUMB antibody (ab4147)

Western blot - NUMB antibody (ab4147)



Predicted band size : 71 kDa


ab4147 staining (0.03µg/ml) of Mouse Brain lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Protocols

  • Recommended WB protocol for ab4147

    • References for Anti-NUMB antibody (ab4147)

    This product has been referenced in:

    • Euskirchen Pet al. NUMB does not impair growth and differentiation status of experimental gliomas. Exp Cell Res : (2011). WB; Human.Read more (PubMed: 21939656) »
    • Oliaro Jet al. Asymmetric cell division of T cells upon antigen presentation uses multiple conserved mechanisms. J Immunol 185:367-75 (2010). ICC/IF; Mouse.Read more (PubMed: 20530266) »

    See all 12 publications for this product

    Publishing research using ab4147? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"