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Products:Neuroscience >> Neurology process >> Notch Pathway
Overview
Product name
NUMB peptide
See all NUMB products (2) ...
Protein description
Synthetic peptide derived from within residues 600 to the C-terminus of Human NUMB. (Note: the amino acid sequence is proprietary).
Protein length
651 amino acids
Expression host
CHO
Properties
Biological activity
ab14141 is the immunising peptide for Numb antibody ab14140 and can be used for blocking studies.
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Information available upon request.
Concentration
Concentration information loading...
Research areas
Stem Cells >> Signaling Pathways >> Notch >> Cytoplasmic
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Notch Pathway
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Western blot
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking
Application notes
BL: Use at a concentration of 0.5 mg/ml.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.
Sequence similarities
Contains 1 PID domain.
Post-translational
modifications
Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Cellular localization
Membrane.
Target information above from: UniProt accessionP49757
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
NUMB peptide images:
Western blot
All lanes : Anti-NUMB antibody (ab14140) at 1 µg/ml
Lane 1 : HEK 293 cells were transfected with pcDNA3.1 vector
Lane 2 : HEK 293 cells were transfected with mNUMB
Lane 3 : HEK 293 cells were transfected with pcDNA3.1 vector and IP'd
Lane 4 : HEK 293 cells were transfected with mNUMB and IP'd
Lane 5 : HEK 293 cells were transfected with pcDNA3.1 vector with NUMB peptide (ab14141) at 0.5 mg/ml
Lane 6 : HEK 293 cells were transfected with mNUMB with NUMB peptide (ab14141) at 0.5 mg/ml
Lysates/proteins at 50 µg per lane.
Performed under reducing conditions.
Predicted band size : 70 kDa
Observed band size : 79 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa (possible IgG).
References for NUMB peptide (ab14141)
ab14141 has not yet been referenced specifically in any publications.
Publishing research using ab14141? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"