Overview

  • Product name
  • Description
    Rabbit polyclonal to NUP188
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 1699 and 1749 of human NUP188 (NP_056169.1)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 196 kDa (predicted molecular weight: 196 kDa).
IP Use at 10 µg/mg of lysate.

Target

  • Relevance
    NUP188 may function as a component of the nuclear pore complex (NPC).
  • Cellular localization
    Nucleus; nuclear pore complex
  • Database links
  • Alternative names
    • BC025526 antibody
    • FLJ21639 antibody
    • hNup188 antibody
    • KIAA0169 antibody
    • mKIAA0169 antibody
    • Nucleoporin 188kDa antibody
    • Nucleoporin NUP188 homolog antibody
    • OTTMUSP00000018197 antibody
    • RP11-167N5.2 antibody
    • RP23-395P6.3 antibody
    • U89435 antibody
    see all

Images

  • All lanes : Anti-NUP188 antibody (ab86601) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 196 kDa
    Observed band size : 196 kDa


    Exposure time : 3 minutes
  • Detection of NUP188 by Western Blot of Immunprecipitate.
    ab86601 at 1µg/ml staining NUP188 in HeLa whole cell lysate immunoprecipitated using ab86601 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

This product has been referenced in:
  • Labade AS  et al. HOXA repression is mediated by nucleoporin Nup93 assisted by its interactors Nup188 and Nup205. Epigenetics Chromatin 9:54 (2016). WB ; Human . Read more (PubMed: 27980680) »
  • Ladopoulos V  et al. The Histone Methyltransferase KMT2B Is Required for RNA Polymerase II Association and Protection from DNA Methylation at the MagohB CpG Island Promoter. Mol Cell Biol 33:1383-93 (2013). Read more (PubMed: 23358417) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Colorectal cancer cell line DLD1)
Total protein in input
500 µg
Immuno-precipitation step
Other - Protein A Dynabeads
Specification
Colorectal cancer cell line DLD1
Username

Ajay Labade

Verified customer

Submitted Jan 31 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (DLD1 cells)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
DLD1 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ajay Labade

Verified customer

Submitted Jan 30 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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