Anti-NUP88 antibody (ab231187)
Key features and details
- Rabbit polyclonal to NUP88
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NUP88 antibody
See all NUP88 primary antibodies -
Description
Rabbit polyclonal to NUP88 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Mouse NUP88 aa 50-300. Expressed in E.Coli. N-terminal tags.
Database link: Q5QNU0 -
Positive control
- WB: Recombinant mouse NUP88 protein; mouse liver lysate; HeLa and HepG2 cell lysates. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Coimmunogen
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 84 kDa.
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ICC/IF |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 84 kDa. |
ICC/IF
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Essential component of nuclear pore complex. -
Tissue specificity
Ubiquitous. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nuclear pore complex. - Information by UniProt
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Database links
- Entrez Gene: 4927 Human
- Entrez Gene: 19069 Mouse
- Entrez Gene: 113929 Rat
- Omim: 602552 Human
- SwissProt: Q99567 Human
- SwissProt: Q8CEC0 Mouse
- SwissProt: O08658 Rat
- Unigene: 584784 Human
see all -
Alternative names
- 88 kDa nucleoporin antibody
- karyoporin antibody
- Nuclear pore complex protein 88 antibody
see all
Images
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NUP88 (green) using ab231187 at 20 μg/ml in ICC/IF. FITC labeled secondary antibody.
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All lanes : Anti-NUP88 antibody (ab231187) at 2 µg/ml
Lane 1 : Mouse liver lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 84 kDa -
Anti-NUP88 antibody (ab231187) at 2 µg/ml + Recombinant mouse NUP88 protein
Predicted band size: 84 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231187 has not yet been referenced specifically in any publications.