• Product nameAnti-NUSAP1 antibody
    See all NUSAP1 primary antibodies
  • Description
    Rabbit polyclonal to NUSAP1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide from a region within amino acids 125 - 175 of Human NUSAP1 (NP_057443.2)

  • Positive control
    • HeLa cell lysate 293T cell lysate



Our Abpromise guarantee covers the use of ab93779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIP: Use at 5-15µg/mg of lysate.
    WB: 1/1000 - 1/5000. Predicted molecular weight: 49 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMicrotubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.
    • Sequence similaritiesBelongs to the NUSAP family.
    • DomainThe KEN box is required for the FZR1-dependent degradation of this protein subsequent to ubiquitination.
    • Post-translational
      Phosphorylated upon DNA damage, probably by ATM or ATR.
      Ubiquitinated. Ubiquitination by FZR1 may lead to proteasome-dependent degradation of this protein.
    • Cellular localizationCytoplasm. Nucleus > nucleolus. Cytoplasm > cytoskeleton > spindle. Chromosome. Found in the cytoplasm and nucleolus during interphase and redistributes to the mitotic spindle in prometaphase (By similarity). Localizes to the mitotic spindle during anaphase and telophase then disappears from around the chromosomes during cytokinesis (By similarity). Localizes to multiple distinct regions of chromosomes throughout mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2610201A12Rik antibody
      • AI481307 antibody
      • ANKT antibody
      • AW547774 antibody
      • BB165529 antibody
      • BM037 antibody
      • FLJ13421 antibody
      • LNP antibody
      • Nucleolar and spindle associated protein 1 antibody
      • Nucleolar and spindle-associated protein 1 antibody
      • Nucleolar protein ANKT antibody
      • NuSAP antibody
      • NUSAP_HUMAN antibody
      • nusap1 antibody
      • PRO0310p1 antibody
      • Q0310 antibody
      • RP23-22A15.4 antibody
      • SAPL antibody
      see all

    Anti-NUSAP1 antibody images

    • All lanes : Anti-NUSAP1 antibody (ab93779) at 0.4 µg/ml

      Lane 1 : HeLa cell lysate at 50 µg
      Lane 2 : HeLa cell lysate at 15 µg
      Lane 3 : HeLa cell lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 49 kDa

      Exposure time : 3 minutes
    • Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with antibody at 10 µg/mg lysate; 20% of immunoprecipitate loaded in each lane
      All lanes : NUSAP1 antibody (ab93779) at 1 µg/ml
      Lane 1 : Lysate immunoprecipitated with ab93779
      Lane 2 : Lysate immunoprecipitated with a control IgG

      Developed using the ECL technique
      Exposure time : 30 seconds

    References for Anti-NUSAP1 antibody (ab93779)

    This product has been referenced in:
    • Xie P  et al. ATM-mediated NuSAP phosphorylation induces mitotic arrest. Biochem Biophys Res Commun 404:413-8 (2011). WB, IP ; Human . Read more (PubMed: 21130744) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Different human cell lines)
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Nupage)
    Loading amount 40 µg
    Specification Different human cell lines
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

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    Submitted Jan 18 2017