The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 5-15µg/mg of lysate.
WB: 1/1000 - 1/5000. Predicted molecular weight: 49 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMicrotubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.
Sequence similaritiesBelongs to the NUSAP family.
DomainThe KEN box is required for the FZR1-dependent degradation of this protein subsequent to ubiquitination.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Ubiquitinated. Ubiquitination by FZR1 may lead to proteasome-dependent degradation of this protein.
Cellular localizationCytoplasm. Nucleus > nucleolus. Cytoplasm > cytoskeleton > spindle. Chromosome. Found in the cytoplasm and nucleolus during interphase and redistributes to the mitotic spindle in prometaphase (By similarity). Localizes to the mitotic spindle during anaphase and telophase then disappears from around the chromosomes during cytokinesis (By similarity). Localizes to multiple distinct regions of chromosomes throughout mitosis.
Nucleolar and spindle associated protein 1 antibody
Nucleolar and spindle-associated protein 1 antibody
Nucleolar protein ANKT antibody
Anti-NUSAP1 antibody images
Western blot - NUSAP1 antibody (ab93779)
All lanes : Anti-NUSAP1 antibody (ab93779) at 0.4 µg/ml
Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 49 kDa
Exposure time : 3 minutes
Immunoprecipitation - NUSAP1 antibody (ab93779)
Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with antibody at 10 µg/mg lysate; 20% of immunoprecipitate loaded in each lane All lanes : NUSAP1 antibody (ab93779) at 1 µg/ml Lane 1 : Lysate immunoprecipitated with ab93779 Lane 2 : Lysate immunoprecipitated with a control IgG
Developed using the ECL technique Exposure time : 30 seconds
References for Anti-NUSAP1 antibody (ab93779)
This product has been referenced in:
Xie P et al. ATM-mediated NuSAP phosphorylation induces mitotic arrest. Biochem Biophys Res Commun404:413-8 (2011).
Read more (PubMed: 21130744) »