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Products:Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nuclear Pore
Anti-NXF1 antibody [53H8] (ab50609)
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab50609 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - NXF1 antibody [53H8] (ab50609)](/ps/datasheet/Images/50/ab50609/ab50609.jpg)
Western blot - NXF1 antibody [53H8] (ab50609)
Anti-NXF1 antibody [53H8] (ab50609) at 0.5 µg/ml + Hela nuclear cell extract at 200 µg/ml
Predicted band size : 70 kDa
Observed band size : 62 kDa (why is the actual band size different from the predicted?)
![Immunocytochemistry/ Immunofluorescence - NXF1 antibody [53H8] (ab50609)](/ps/datasheet/images/50/ab50609/NXF1-Primary-antibodies-ab50609-3.jpg)
Immunocytochemistry/ Immunofluorescence - NXF1 antibody [53H8] (ab50609)
ab50609 staining NXF1 in HeLa cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100. The primary antibody was diluted 1/1000 in 3% BSA in PBS and incubated with the sample for 1 hour at 25ºC. The secondary antibody was Alexa Fluor® 568-conjugated goat anti-mouse polyclonal, diluted 1/1000. DAPI was used for nuclear counterstain.
This image is courtesy of an Anonymous Abreview.
![NXF1 antibody [53H8] for Immunocytochemistry/ Immunofluorescence in Human (50609)](/ps/datasheet/images/50/ab50609/NXF1-Primary-antibodies-ab50609-2.jpg)
NXF1 antibody [53H8] for Immunocytochemistry/ Immunofluorescence in Human (50609)
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