![Western blot - NXF1 antibody [53H8] (ab50609) image](#ab50609.jpg)
![Immunocytochemistry/ Immunofluorescence - NXF1 antibody [53H8] (ab50609) image](#NXF1-Primary-antibodies-ab50609-3.jpg)
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Products:Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nuclear Pore
Overview
Product name
Anti-NXF1 antibody [53H8]
See all NXF1 products (3) ...
Description
Mouse monoclonal [53H8] to NXF1
Tested applications
Dot Blot, ICC/IF, WB, IP, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
TAP (Tip Associated Protein)
Positive control
HeLa nuclear cell extract
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20ºC.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
53H8
Myeloma
Sp2/0
Isotype
IgG1
Research areas
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nucleoplasm
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nuclear Pore
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use a concentration of 0.5 - 1 µg/ml.
ICC/IF: Use at an assay dependent dilution.
WB: Use a concentration of 0.5 - 1 µg/ml.Predicted molecular weight: 70 kDa.
IP: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
Target
Function
Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with THOC4 and THOC5.
Tissue specificity
Expressed ubiquitously.
Sequence similarities
Belongs to the NXF family.
Contains 4 LRR (leucine-rich) repeats.
Contains 1 NTF2 domain.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 TAP-C domain.
Domain
The minimal CTE binding domain consists of an RNP-type RNA binding domain (RBD) and leucine-rich repeats.
The nucleoporin binding domain consists of a NTF2 domain (also called NTF2-like domain) and a TAP-C domain (also called UBA-like domain). It has 2 nucleoporin-FG-repeats binding sites (one in the NTF2 and the other in the TAP-C domain) which contribute to nucleoporin association and act synergistically to export cellular mRNAs.
The NTF2 domain is functional only in the presence of NXT1 and is essential for the export of mRNA from the nucleus.
The TAP-C domain mediates direct interactions with nucleoporin-FG-repeats and is necessary and sufficient for localization of NXF1 to the nuclear rim. The conserved loop 594-NWD-596 of the TAP-C domain has a critical role in the interaction with nucleoporins.
The leucine-rich repeats are essential for the export of mRNA from the nucleus.
The RNA-binding domain is a non-canonical RNP-type domain.
Cellular localization
Nucleus > nucleoplasm. Nucleus speckle. Cytoplasm. Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA.
Target information above from: UniProt accessionQ9UBU9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp667O0311 antibody
- DmNXF1 antibody
- MEX67 antibody
see all
Database links
- Entrez Gene: 10482 Human
- Omim: 602647 Human
- SwissProt: Q9UBU9 Human
- Unigene: 523739 Human
- Unigene: 601546 Human
Anti-NXF1 antibody [53H8] images:
Western blot - NXF1 antibody [53H8] (ab50609)
![Western blot - NXF1 antibody [53H8] (ab50609)](/ps/datasheet/Images/50/ab50609/ab50609.jpg)
Anti-NXF1 antibody [53H8] (ab50609) at 0.5 µg/ml + Hela nuclear cell extract at 200 µg/ml
Predicted band size : 70 kDa
Observed band size : 62 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - NXF1 antibody [53H8] (ab50609)
![Immunocytochemistry/ Immunofluorescence - NXF1 antibody [53H8] (ab50609)](/ps/datasheet/images/50/ab50609/NXF1-Primary-antibodies-ab50609-3.jpg)
ab50609 staining NXF1 in HeLa cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100. The primary antibody was diluted 1/1000 in 3% BSA in PBS and incubated with the sample for 1 hour at 25°C. The secondary antibody was Alexa Fluor® 568-conjugated goat anti-mouse polyclonal, diluted 1/1000. DAPI was used for nuclear counterstain.
This image is courtesy of an Anonymous Abreview.
References for Anti-NXF1 antibody [53H8] (ab50609)
This product has been referenced in:
- Read EK & Digard P Individual influenza A virus mRNAs show differential dependence on cellular NXF1/TAP for their nuclear export. J Gen Virol 91:1290-301 (2010). WB, in situ hybridisation; Human.Read more (PubMed: 20071484) »
- Wang Wet al. Imaging and characterizing influenza A virus mRNA transport in living cells. Nucleic Acids Res 36:4913-28 (2008). ICC/IF, IP.Read more (PubMed: 18653528) »
See all 2 publications for this product
Publishing research using ab50609? Please let us know so that we can cite the reference in this datasheet
![NXF1 antibody [53H8] for Immunocytochemistry/ Immunofluorescence in Human (50609)](/ps/datasheet/images/50/ab50609/NXF1-Primary-antibodies-ab50609-2.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"