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Western blot - NXF1 antibody (ab43421)
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Immunocytochemistry/ Immunofluorescence - NXF1 antibody (ab43421)
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Immunocytochemistry/ Immunofluorescence - NXF1 antibody (ab43421)
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Product Name
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NXF1 antibody
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See all NXF1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to NXF1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human NXF1. (Peptide available as ab43638.)
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Reacts with
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Hu
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Predicted to react
(species key)
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Cow, Orngt
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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ab43421 gave a positive signal in the following Human Tissue Lysates: Brain, Testis, Heart and Skeletal muscle.
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Cellular localization
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Nucleus (nucleoplasm). Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex (shuttles between both).
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Research areas
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Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nucleoplasm
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nuclear Pore
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Relevance
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NXF1 (Nuclear Export Factor 1)is a 70 kDa protein involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and is thought to be a principal export receptor for cellular mRNA, from the nucleus to the cytoplasm. NXF1 is primarily located in the nucleoplasm but it can also be found in association with both the cytoplasmic and the nucleoplasmic ends of the nuclear pore complex. It contains an N-terminal nuclear localization and export region (NLS-NES), a central RNA binding domain (RBD), and a C-terminal portion that is required for nuclear localization and nuclear rim association. It is expressed ubiquitously.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NXF1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NXF1 antibody images, references, products related to ab43421 and other tools.
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NXF1 antibody images:
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 Immunocytochemistry/ Immunofluorescence - NXF1 antibody (ab43421)
ICC/IF image of ab43421 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab43421, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Immunocytochemistry/ Immunofluorescence - NXF1 antibody (ab43421)
ab43421 (1/200) staining NXF1 in HeLa cells (green). cells were fixed in paraformaldehyde and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details. This image is courtesy of an Abreview submitted by Dr Kirk McManus
See Abreview
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Search PubMed (MEDLINE) for references to NXF1
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NXF1 antibody - more information
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Customer reviews (feedback) regarding NXF1 antibody |
Customer FAQs regarding NXF1 antibody |
Protocols for NXF1 antibody |
Price and availability of products related to NXF1 antibody |
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NXF1 antibody - related products:
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NXF1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about NXF1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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