Overview
- Product nameAnti-NYS48 antibodySee all NYS48 primary antibodies ...
- DescriptionRabbit polyclonal to NYS48
- Tested applicationsWB, IHC-P, ICC/IF more details
- Species reactivityReacts with: Human
- Immunogen
The immunogen is a recombinant protein corresponding to a region within amino acids 216-410 of human NYS48
- Positive controlK562 and HeLa lysates
Properties
- FormLiquid
- Storage instructionsStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab95970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/2000. Predicted molecular weight: 45 kDa. |
| IHC-P | IHC-P: 1/50 - 1/500. |
| ICC/IF | ICC/IF: 1/100 - 1/200. |
Target
- RelevanceNYS48 belongs to the HAUS8 family. It is required for stabilizing spindle integrity and chromosomal integrity.
- Cellular localizationCytoplasm, cytoskeleton, Microtubule. Note: During interphase, primarily cytoplasmic and associates with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, additionally associates with the spindle midzone and midbody, respectively.
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Database links
- Entrez Gene: 93323 Human
- Entrez Gene: 93323 Human
- SwissProt: Q9BT25 Human
- SwissProt: Q9BT25 Human
- Unigene: 404088 Human
Anti-NYS48 antibody images
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Western blot - NYS48 antibody (ab95970)All lanes : Anti-NYS48 antibody (ab95970) at 1/500 dilution
Lane 1 : His-Hice 1 (2x)
Lane 2 : His-Hice 1 (1x)
Predicted band size : 45 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NYS48 antibody (ab95970)Immunohistochemical analysis of paraffin-embedded human lymph tissue using ab95970 at 1/50 dilution. (Image 20x) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NYS48 antibody (ab95970)Immunohistochemical analysis of paraffin-embedded human lymph tissue using ab95970 at 1/50 dilution. (Image 40x) -
Immunocytochemistry/ Immunofluorescence - NYS48 antibody (ab95970)Immunoflourescenc analysis of human osteosarcoma cell line U2OS using ab95970 against NYS48 (1), alpha tubulin (2) and merged with DAP1 (3) at 1/50-1/200 dilution
References for Anti-NYS48 antibody (ab95970)
ab95970 has not yet been referenced specifically in any publications.
