Overview

  • Product nameAnti-Nab2 antibodySee all Nab2 primary antibodies ...
  • Description
    Rabbit polyclonal to Nab2
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee
  • Immunogen

    A region within synthetic peptide: MHRAPSPTAE QPPGGGDSAR RTLQPRLKPS ARAMALPRTL GELQLYRVLQ, corresponding to N terminal amino acids 1-50 of Human Nab2

  • Positive control
    • HepG2 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab66604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

Target

  • FunctionActs as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.
  • Tissue specificityWidely expressed at low levels. Highly expressed in melanoma cell lines.
  • Sequence similaritiesBelongs to the NAB family.
  • DomainThe NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
    The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
  • Cellular localizationNucleus. Isoform 2 is not localized to the nucleus.
  • Target information above from: UniProt accession Q15742 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • EGR 1 binding protein 2 antibody
    • EGR-1-binding protein 2 antibody
    • EGR1 binding protein 2 antibody
    • MADER antibody
    • Melanoma associated delayed early response protein antibody
    • Melanoma-associated delayed early response protein antibody
    • MGC75085 antibody
    • Nab 2 antibody
    • nab2 antibody
    • NAB2_HUMAN antibody
    • NGFI A binding protein 2 (EGR1 binding protein 2) antibody
    • NGFI A binding protein 2 antibody
    • NGFI-A-binding protein 2 antibody
    • Protein MADER antibody
    see all

Anti-Nab2 antibody images

  • All lanes : Anti-Nab2 antibody (ab66604) at 1/1.25 dilution (in 5% skim milk / PBS buffer)

    Lane 1 : marker
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 48,62 kDa (why is the actual band size different from the predicted?)

References for Anti-Nab2 antibody (ab66604)

ab66604 has not yet been referenced specifically in any publications.

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