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Western blot - Nac1 antibody [3] (ab81987)
Western blot - Nac1 antibody [3] (ab81987)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nac1 antibody [3] (ab81987)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nac1 antibody [3] (ab81987)
(enlarge)


Product Name 

Nac1 antibody [3]

 

See all Nac1 antibodies (4)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [3] to Nac1

Immunogen 

Recombinant human NAC1 protein.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ICC/IF, IHC-P, IP, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/100.
IHC-P: 1/100.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.3 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa nuclear lysate
Human ovarian cancer tissue

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Stem Cells >> Embryonic Stem Cells >> Intracellular
Chromatin and Nuclear Signaling >> Transcription >> Other factors

Relevance 

Nac1 is a transcriptional repressor with a BTB domain. In humans the protein is upregulated in a number of carcinomas and is localized in discrete nuclear bodies that correlate with tumor recurrence.

 

Nac1 antibody [3] (ab81987)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

112939    (Human)

SwissProt

    

Q96RE7    (Human)

Unigene

    

531614    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

3

Isotype 

IgG

Purity 

Protein G purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: Tris glycine, 150mM Sodium chloride

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Protein G purified Mouse ascites

Form 

Liquid

Concentration 

0.820 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Nac1 antibody [3] is on this datasheet. But please do contact us if you would like any reassurance!


See below for Nac1 antibody [3] images, references, products related to ab81987 and other tools.

 

Nac1 antibody [3] images:

Product image  Western blot - Nac1 antibody [3] (ab81987)

Western blot - Nac1 antibody [3] (ab81987)

Nac1 antibody [3] (ab81987) at 0.3 µg/ml + HeLa nuclear extracts

Predicted band size : 57 kDa
Observed band size : 57 kDa

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nac1 antibody [3] (ab81987)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nac1 antibody [3] (ab81987)

ab81987 at a 1/100 dilution, staining human Nac1 in ovarian cancer using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

 
 

Search PubMed (MEDLINE) for references to Nac1

 

Nac1 antibody [3] - more information

Customer reviews (feedback) regarding Nac1 antibody [3]

Customer FAQs regarding Nac1 antibody [3]

Protocols for Nac1 antibody [3]

Price and availability of products related to Nac1 antibody [3]

 

Nac1 antibody [3] - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for Nac1 antibody [3]

ab14655

  

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

 

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Other Nac1 Antibodies

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Nac1 antibody [3] - other tools:

Contact Abcam with a Technical Enquiry about Nac1 antibody [3]

 

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