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Anti-Nac1 antibody [3] (ab81987)

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Overview

Product name

Anti-Nac1 antibody [3]
See all Nac1 products (5) ...

Description

Mouse monoclonal [3] to Nac1

Tested applications

WB, IHC-P, ICC/IF, IPmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant human NAC1 protein.

Positive control

HeLa nuclear lysate Human ovarian cancer tissue

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: Tris glycine, 150mM Sodium chloride

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

Protein G purified Mouse ascites

Clonality

Monoclonal

Clone number

3

Isotype

IgG

Research areas

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Stem Cells >> Embryonic Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Transcription >> Other factors

  • Western blot - Nac1 antibody [3] (ab81987)Western blot - Nac1 antibody [3] (ab81987) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nac1 antibody [3] (ab81987)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nac1 antibody [3] (ab81987) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab81987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 0.3 µg/mlDetects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/100

    IHC-P: 1/100

  • ICC/IF

     ICC/IF: 1/100

    ICC/IF: 1/100

  • ShowHide

    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution.

Target

Function

Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines.

Tissue specificity

Overexpressed in several types of carcinomas including ovarian serous carcinomas. Expression levels positively correlate with tumor recurrence in ovarian serous carcinomas, and intense immunoreactivity in primary ovarian tumors predicts early recurrence. Up-regulated in ovarian carcinomas after chemotherapy, suggesting a role in development of chemotherapy resistance in ovarian cancer.

Sequence similarities

Contains 1 BEN domain.
Contains 1 BTB (POZ) domain.

Cellular localization

Nucleus. Cytoplasm. Distribution in the cytoplasm is dependent on phosphorylation.

Target information above from: UniProt accessionQ96RE7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • BEND8 antibody
  • BTB Domain Containing 14B antibody
  • BTB/POZ domain-containing protein 14B antibody
  • btbd14b antibody
  • FLJ37383 antibody
  • NAC-1 antibody
  • NAC1 antibody
  • Nacc1 antibody
  • NACC1_HUMAN antibody
  • Nucleus accumbens-associated protein 1 antibody
see all

Database links

Anti-Nac1 antibody [3] images:

  Western blot - Nac1 antibody [3] (ab81987)

Western blot - Nac1 antibody [3] (ab81987)

Anti-Nac1 antibody [3] (ab81987) at 0.3 µg/ml + HeLa nuclear extracts

Predicted band size : 57 kDa
Observed band size : 57 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nac1 antibody [3] (ab81987)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nac1 antibody [3] (ab81987)

ab81987 at a 1/100 dilution, staining human Nac1 in ovarian cancer using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

References for Anti-Nac1 antibody [3] (ab81987)

ab81987 has not yet been referenced specifically in any publications.

Publishing research using ab81987? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"