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Immunocytochemistry/ Immunofluorescence - Nac1 antibody (ab29047)
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Western blot - Nac1 antibody (ab29047)
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Western blot - Nac1 antibody (ab29047)
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Product Name
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Nac1 antibody
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See all Nac1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Nac1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Mouse Nac1. (Peptide available as ab30604.)
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Reacts with
(species key)
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Ms, Rat
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/250. Detects bands of approximately 57 and 60 kDa (predicted molecular weight: 57 kDa).
We are unsure as to the nature of the doublet. It is likely that the doublet is caused by the presence of processed and unprocessed forms of Nac1. Both bands are blocked by addition of the immunizing peptide (ab30604).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following lysates:
F9 (Mouse embryonic carcinoma cell line) Whole Cell
IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell
Brain (Rat) Tissue Lysate - normal tissue
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Stem Cells >> Embryonic Stem Cells >> Intracellular
Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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Nac1 is a transcriptional repressor with a BTB domain. In humans the protein is upregulated in a number of carcinomas and is localized in discrete nuclear bodies that correlate with tumor recurrence.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.400 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Nac1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Nac1 antibody images, references, products related to ab29047 and other tools.
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Nac1 antibody images:
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 Immunocytochemistry/ Immunofluorescence - Nac1 antibody (ab29047)
ICC/IF image of ab29047 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab29047, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
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Western blot - Nac1 antibody (ab29047)
All lanes : Nac1 antibody (ab29047) at 1/250 dilution
Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193)
Lane 2 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate (ab27202)
Lane 3 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193) with Nac1 peptide (ab30604) at 1 µg/ml
Lane 4 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate (ab27202) with Nac1 peptide (ab30604) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 57 kDa
Observed band size : 57 kDa
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
We are unsure as to the nature of the doublet. It is likely that the doublet is caused by the presence of processed and unprocessed forms of Nac1. Both bands are blocked by addition of the immunizing peptide (ab30604).
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Western blot - Nac1 antibody (ab29047)
Nac1 antibody (ab29047) at 1/250 dilution + Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 57 kDa
Observed band size : 57 kDa
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Search PubMed (MEDLINE) for references to Nac1
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Nac1 antibody - more information
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Customer reviews (feedback) regarding Nac1 antibody |
Customer FAQs regarding Nac1 antibody |
Protocols for Nac1 antibody |
Price and availability of products related to Nac1 antibody |
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Nac1 antibody - related products:
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Nac1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Nac1 antibody |
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