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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-Nac1 antibody
See all Nac1 products (5) ...
Description
Rabbit polyclonal to Nac1
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Mouse Nac1.
(Peptide available as ab30604.)
Positive control
This antibody gave a positive signal in the following lysates: F9 (Mouse embryonic carcinoma cell line) Whole Cell IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Brain (Rat) Tissue Lysate - normal tissue
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab29047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
ICC/IF
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/250. Detects bands of approximately 57 and 60 kDa (predicted molecular weight: 57 kDa).
We are unsure as to the nature of the doublet. It is likely that the doublet is caused by the presence of processed and unprocessed forms of Nac1. Both bands are blocked by addition of the immunizing peptide (ab30604).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines.
Tissue specificity
Overexpressed in several types of carcinomas including ovarian serous carcinomas. Expression levels positively correlate with tumor recurrence in ovarian serous carcinomas, and intense immunoreactivity in primary ovarian tumors predicts early recurrence. Up-regulated in ovarian carcinomas after chemotherapy, suggesting a role in development of chemotherapy resistance in ovarian cancer.
Sequence similarities
Contains 1 BEN domain.
Contains 1 BTB (POZ) domain.
Cellular localization
Nucleus. Cytoplasm. Distribution in the cytoplasm is dependent on phosphorylation.
Target information above from: UniProt accessionQ96RE7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BEND8 antibody
- BTB Domain Containing 14B antibody
- BTB/POZ domain-containing protein 14B antibody
see all
Database links
- Entrez Gene: 112939 Human
- Entrez Gene: 66830 Mouse
- Entrez Gene: 171454 Rat
- Omim: 610672 Human
- SwissProt: Q96RE7 Human
- SwissProt: Q7TSZ8 Mouse
- SwissProt: Q80X97 Mouse
- SwissProt: Q9CZ72 Mouse
see all
Anti-Nac1 antibody images:
Immunocytochemistry/ Immunofluorescence - Nac1 antibody (ab29047)
ICC/IF image of ab29047 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab29047, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
Western blot - Nac1 antibody (ab29047)
All lanes : Anti-Nac1 antibody (ab29047) at 1/250 dilution
Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193)
Lane 2 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate (ab27202)
Lane 3 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193) with Nac1 peptide (ab30604) at 1 µg/ml
Lane 4 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate (ab27202) with Nac1 peptide (ab30604) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 57 kDa
Observed band size : 57 kDa
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
We are unsure as to the nature of the doublet. It is likely that the doublet is caused by the presence of processed and unprocessed forms of Nac1. Both bands are blocked by addition of the immunizing peptide (ab30604).
Western blot - Nac1 antibody (ab29047)
Anti-Nac1 antibody (ab29047) at 1/250 dilution + Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 57 kDa
Observed band size : 57 kDa
References for Anti-Nac1 antibody (ab29047)
ab29047 has not yet been referenced specifically in any publications.
Publishing research using ab29047? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"