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Anti-Nac1 antibody (ab29047)

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Overview

Product name

Anti-Nac1 antibody
See all Nac1 products (5) ...

Description

Rabbit polyclonal to Nac1

Tested applications

WB, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Mouse Nac1.

(Peptide available as ab30604.)

Positive control

This antibody gave a positive signal in the following lysates: F9 (Mouse embryonic carcinoma cell line) Whole Cell IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Brain (Rat) Tissue Lysate - normal tissue

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Immunocytochemistry/ Immunofluorescence - Nac1 antibody (ab29047)Immunocytochemistry/ Immunofluorescence - Nac1 antibody (ab29047) image (enlarge)

  • Western blot - Nac1 antibody (ab29047)Western blot - Nac1 antibody (ab29047) image (enlarge)

  • Western blot - Nac1 antibody (ab29047)Western blot - Nac1 antibody (ab29047) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab29047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/250. Detects bands of approximately 57 and 60 kDa (predicted molecular weight: 57 kDa).

We are unsure as to the nature of the doublet. It is likely that the doublet is caused by the presence of processed and unprocessed forms of Nac1. Both bands are blocked by addition of the immunizing peptide (ab30604).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines.

Tissue specificity

Overexpressed in several types of carcinomas including ovarian serous carcinomas. Expression levels positively correlate with tumor recurrence in ovarian serous carcinomas, and intense immunoreactivity in primary ovarian tumors predicts early recurrence. Up-regulated in ovarian carcinomas after chemotherapy, suggesting a role in development of chemotherapy resistance in ovarian cancer.

Sequence similarities

Contains 1 BEN domain.
Contains 1 BTB (POZ) domain.

Cellular localization

Nucleus. Cytoplasm. Distribution in the cytoplasm is dependent on phosphorylation.

Target information above from: UniProt accessionQ96RE7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • BEND8 antibody
  • BTB Domain Containing 14B antibody
  • BTB/POZ domain-containing protein 14B antibody
  • btbd14b antibody
  • btbd14b antibody
  • FLJ37383 antibody
  • NAC-1 antibody
  • NAC1 antibody
  • Nacc1 antibody
  • NACC1_HUMAN antibody
  • Nucleus accumbens-associated protein 1 antibody
see all

Anti-Nac1 antibody images:

  Immunocytochemistry/ Immunofluorescence - Nac1 antibody (ab29047)

Immunocytochemistry/ Immunofluorescence - Nac1 antibody (ab29047)

ICC/IF image of ab29047 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab29047, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

  Western blot - Nac1 antibody (ab29047)

Western blot - Nac1 antibody (ab29047)

All lanes : Anti-Nac1 antibody (ab29047) at 1/250 dilution

Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193)
Lane 2 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate (ab27202)
Lane 3 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193) with Nac1 peptide (ab30604) at 1 µg/ml
Lane 4 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate (ab27202) with Nac1 peptide (ab30604) at 1 µg/ml

Lysates/proteins at 20 µg per lane.

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 57 kDa
Observed band size : 57 kDa
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

We are unsure as to the nature of the doublet. It is likely that the doublet is caused by the presence of processed and unprocessed forms of Nac1. Both bands are blocked by addition of the immunizing peptide (ab30604).

  Western blot - Nac1 antibody (ab29047)

Western blot - Nac1 antibody (ab29047)

Anti-Nac1 antibody (ab29047) at 1/250 dilution + Brain (Rat) Tissue Lysate - normal tissue at 10 µg

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 57 kDa
Observed band size : 57 kDa

References for Anti-Nac1 antibody (ab29047)

ab29047 has not yet been referenced specifically in any publications.

Publishing research using ab29047? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"