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Western blot - Nac1 antibody (ab60264)
Western blot - Nac1 antibody (ab60264)
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Product Name 

Nac1 antibody

 

See all Nac1 antibodies (4)...

Product type 

Primary antibodies

Description 

Goat polyclonal to Nac1

Immunogen 

Synthetic peptide: KTEQQESDSVQC, corresponding to internal sequence amino acids 167-178 of Human Nac1
BLAST 'KTEQQESDSVQC' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Hu

Predicted to react 
(species key)

Chmp, Cow, Dog, Hrs, Op, RMk
(Due to sequence homology)

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
Peptide ELISA: antibody detection limit dilution 1/2000.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Bone Marrow lysate.

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Stem Cells >> Embryonic Stem Cells >> Intracellular
Chromatin and Nuclear Signaling >> Transcription >> Other factors

Relevance 

Nac1 is a transcriptional repressor with a BTB domain. In humans the protein is upregulated in a number of carcinomas and is localized in discrete nuclear bodies that correlate with tumor recurrence.

 

Alternative names Nac1 antibody (ab60264)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

112939    (Human)

SwissProt

    

Q96RE7    (Human)

Unigene

    

531614    (Human)

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Nac1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Nac1 antibody images, references, products related to ab60264 and other tools.

 

Nac1 antibody images:

Product image  Western blot - Nac1 antibody (ab60264)

Western blot - Nac1 antibody (ab60264)

ab60264 antibody (0.5µg/ml) staining of Nac1 in Human Bone Marrow lysate (35µg protein in RIPA buffer)

Predicted band size : 57 kDa
Observed band size : 57 kDa


Primary incubation was 1 hour. Detected by chemiluminescence.

 
 

Search PubMed (MEDLINE) for references to Nac1

 

Nac1 antibody - more information

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Lysates

 

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ab7004

  

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ab27245

  

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