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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Anti-Nac1 antibody
See all Nac1 products (5) ...
Goat polyclonal to Nac1
ELISA, WBmore details
Reacts with
Human
Predicted to work with
Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Opossum
Synthetic peptide: KTEQQESDSVQC, corresponding to internal sequence amino acids 167-178 of Human Nac1
KTEQQESDSV QC
Bone Marrow lysate.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Polyclonal
IgG
Western blot - Nac1 antibody (ab60264)
(enlarge)
Our Abpromise guarantee covers the use of ab60264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: antibody detection limit dilution 1/2000.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines.
Overexpressed in several types of carcinomas including ovarian serous carcinomas. Expression levels positively correlate with tumor recurrence in ovarian serous carcinomas, and intense immunoreactivity in primary ovarian tumors predicts early recurrence. Up-regulated in ovarian carcinomas after chemotherapy, suggesting a role in development of chemotherapy resistance in ovarian cancer.
Contains 1 BEN domain.
Contains 1 BTB (POZ) domain.
Nucleus. Cytoplasm. Distribution in the cytoplasm is dependent on phosphorylation.
Target information above from: UniProt accessionQ96RE7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - Nac1 antibody (ab60264)

ab60264 antibody (0.5µg/ml) staining of Nac1 in Human Bone Marrow lysate (35µg protein in RIPA buffer)
Predicted band size : 57 kDa
Observed band size : 57 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
ab60264 has not yet been referenced specifically in any publications.
Publishing research using ab60264? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
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