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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-Nac1 antibody
See all Nac1 products (5) ...
Description
Goat polyclonal to Nac1
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Opossum
Immunogen
Synthetic peptide: KTEQQESDSVQC, corresponding to internal sequence amino acids 167-178 of Human Nac1
KTEQQESDSV QC
Positive control
Bone Marrow lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - Nac1 antibody (ab60264)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab60264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
Peptide ELISA: antibody detection limit dilution 1/2000.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines.
Tissue specificity
Overexpressed in several types of carcinomas including ovarian serous carcinomas. Expression levels positively correlate with tumor recurrence in ovarian serous carcinomas, and intense immunoreactivity in primary ovarian tumors predicts early recurrence. Up-regulated in ovarian carcinomas after chemotherapy, suggesting a role in development of chemotherapy resistance in ovarian cancer.
Sequence similarities
Contains 1 BEN domain.
Contains 1 BTB (POZ) domain.
Cellular localization
Nucleus. Cytoplasm. Distribution in the cytoplasm is dependent on phosphorylation.
Target information above from: UniProt accessionQ96RE7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BEND8 antibody
- BTB Domain Containing 14B antibody
- BTB/POZ domain-containing protein 14B antibody
see all
Database links
- Entrez Gene: 112939 Human
- Omim: 610672 Human
- SwissProt: Q96RE7 Human
- Unigene: 531614 Human
Anti-Nac1 antibody images:
Western blot - Nac1 antibody (ab60264)
ab60264 antibody (0.5µg/ml) staining of Nac1 in Human Bone Marrow lysate (35µg protein in RIPA buffer)
Predicted band size : 57 kDa
Observed band size : 57 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-Nac1 antibody (ab60264)
ab60264 has not yet been referenced specifically in any publications.
Publishing research using ab60264? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"