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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-Nac1 antibody
See all Nac1 products (5) ...
Description
Rabbit polyclonal to Nac1
Tested applications
ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide selected from the centre region of human Nac1; conjugated to KLH
Positive control
Human hepatocarcinoma tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nac1 antibody (ab80144)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab80144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
IHC-P: 1/10 - 1/50.
Target
Function
Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines.
Tissue specificity
Overexpressed in several types of carcinomas including ovarian serous carcinomas. Expression levels positively correlate with tumor recurrence in ovarian serous carcinomas, and intense immunoreactivity in primary ovarian tumors predicts early recurrence. Up-regulated in ovarian carcinomas after chemotherapy, suggesting a role in development of chemotherapy resistance in ovarian cancer.
Sequence similarities
Contains 1 BEN domain.
Contains 1 BTB (POZ) domain.
Cellular localization
Nucleus. Cytoplasm. Distribution in the cytoplasm is dependent on phosphorylation.
Target information above from: UniProt accessionQ96RE7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BEND8 antibody
- BTB Domain Containing 14B antibody
- BTB/POZ domain-containing protein 14B antibody
see all
Database links
- Entrez Gene: 112939 Human
- Omim: 610672 Human
- SwissProt: Q96RE7 Human
- Unigene: 531614 Human
Anti-Nac1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nac1 antibody (ab80144)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab80144 at a dilution of 1/10, peroxidase-conjugated to the secondary antibody, followed by DAB staining.
References for Anti-Nac1 antibody (ab80144)
ab80144 has not yet been referenced specifically in any publications.
Publishing research using ab80144? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"