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Anti-Nanog antibody [1E6C4] (ab76586)

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Overview

  • Product nameAnti-Nanog antibody [1E6C4]See all Nanog primary antibodies ...
  • Description
    Mouse monoclonal [1E6C4] to Nanog
  • Tested applicationsWB, ELISA, Sandwich ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Trx-tagged recombinant fragment, corresponding to amino acids 20-166 of Human Nanog expressed in E.Coli strain BL21 (DE3)

  • Positive controlNanog-transfected CHO-K1 cells lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab76586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/2000. Predicted molecular weight: 35 kDa.
ELISA ELISA: 1/10000.
Sandwich ELISA sELISA: Use a concentration of 0.2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Nanog - ChIP Grade (ab21624). For sandwich ELISA, use this antibody as Capture at 0.2 µg/ml with Rabbit polyclonal to Nanog - ChIP Grade (ab21624) as Detection.

Target

  • FunctionTranscription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • Tissue specificityExpressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • Sequence similaritiesBelongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stageExpressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q9H9S0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Embryonic stem cell specific homeobox protein (Nanog) antibodyENK antibodyFLJ12581 antibody
        FLJ40451 antibodyhNanog antibodyHomeobox protein NANOG antibodyHomeobox transcription factor Nanog antibodyNANOG antibodyNanog homeobox antibodyNANOG_HUMAN antibody
      see all

    Anti-Nanog antibody [1E6C4] images

    • Sandwich ELISA - Nanog antibody [1E6C4] (ab76586)
      Sandwich ELISA - Nanog antibody [1E6C4] (ab76586)
      Standard Curve for Nanog (Analyte: Nanog protein (ab50053)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [1E6C4] to Nanog (ab76586) at 0.2 ug/ml and Detector Antibody Rabbit polyclonal to Nanog - ChIP Grade (ab21624) at 0.5 ug/ml.
    • Western blot - Nanog antibody [1E6C4] (ab76586)
      Western blot - Nanog antibody [1E6C4] (ab76586)
      All lanes : Anti-Nanog antibody [1E6C4] (ab76586) at 1/500 dilution

      Lane 1 : Lysate from CHO-K1 cells transfected with truncated Trx-tagged Nanog (aa 20-166)
      Lane 2 : Lysate from CHO-K1 cells transfected with GST-tagged truncated Nanog (aa 20-166)
      Lane 3 : Lysate from CHO-K1 cells transfected with full length (aa 1-305) Nanog-hIgGFc


      Predicted band size : 35 kDa

    Protocols

    References for Anti-Nanog antibody [1E6C4] (ab76586)

    ab76586 has not yet been referenced specifically in any publications.

    Publishing research using ab76586 ? Please let us know so that we can cite the reference in this datasheet

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    I have a question in relation to the antibody ab76586(Nanog) Are there any control tissue sections, which were shown to be positive nuclear one? Really need all tissues also positive nucleus? Thank you.

    Thank you for contacting us. On the datasheet for ab76586 we state that Nanog-transfected CHO-K1 cells lysate are a suitable positive control. Reviewing protein expression databases such as Human protein atlas and EBI, lung tissue would be a suitab...

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