Products:Stem Cells >> Embryonic Stem Cells >> Intracellular
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ab50053 |
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ab21624 has been referenced in 19 publications.
Publishing research using ab21624? Please let us know so that we can cite the reference in this datasheet
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Standard Curve for Nanog (Analyte:
ab21624 at a 1/1000 dilution staining Nanog in human embryonic stem cells by ICC. An antibody towards Oct4, which has an identical staining pattern to ab21624, was used as a positive control.
All lanes : Anti-Nanog antibody - ChIP Grade (ab21624) at 1/1000 dilution
Lane 1 : whole cell extract of mouse 3T3 cells expressing mouse Nanog.
Lane 2 : whole cell extract of mouse 3T3 cells expressing human Nanog.
Predicted band size : 34 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
ab21624 recognizes human Nanog but does not recognize mouse Nanog in Western Blot.
ab21624 staining Nanog in Human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20ºC; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/500 in PBS) for 12 hours at 4ºC. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.
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