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Products:Stem Cells >> Embryonic Stem Cells >> Intracellular
Overview
Product name
Anti-Nanog antibody - ChIP Grade
See all Nanog products (22) ...
Description
Rabbit polyclonal to Nanog - ChIP Grade
Tested applications
IHC-P, ICC, Flow Cyt, ICC/IF, WB, Sandwich ELISA, ChIPmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse
Immunogen
Synthetic peptide: CGPEENYPSLQMSSAEMPHTE, corresponding to amino acids 29-49 of Human Nanog.
CGPEENYPSL QMSSAEMPHT E
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Cell cycle >> Cell differentiation
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> SMADs
Stem Cells >> Embryonic Stem Cells >> Intracellular
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution.
ICC
ICC: 1/150 - 1/700.ICC: 1/150 - 1/700.
Flow Cyt: Use at an assay dependent dilution.
ICC/IF: 1/1000
WB: 1/300 - 1/2000.Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).(Some users report a stronger signal after blocking and diluting antibodies with 1% BSA instead of 5% milk.)
sELISA: Use a concentration of 0.5 µg/ml Can be paired for Sandwich ELISA with Mouse monoclonal [1E6C4] to Nanog (ab76586). (For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1E6C4] to Nanog (ab76586) as Capture.)
ChIP: Use at an assay dependent dilution. (PubMed: 18710938)
Target
Function
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
Tissue specificity
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
Sequence similarities
Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain.
Developmental stage
Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9H9S0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- hNanog antibody
see all
Database links
- Entrez Gene: 100293888 Human
- Entrez Gene: 79923 Human
- Omim: 607937 Human
- SwissProt: Q9H9S0 Human
- Unigene: 635882 Human
Anti-Nanog antibody - ChIP Grade images:
Sandwich ELISA - Nanog antibody - ChIP Grade (ab21624)
Standard Curve for Nanog (Analyte: Nanog protein (ab50053)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [1E6C4] to Nanog (ab76586) at 0.2 ug/ml and Detector Antibody Rabbit polyclonal to Nanog - ChIP Grade (ab21624) at 0.5 ug/ml.
Immunofluorescence - Nanog antibody - Embryonic Stem Cell Marker (ab21624)
ab21624 at a 1/1000 dilution staining Nanog in human embryonic stem cells by ICC. An antibody towards Oct4, which has an identical staining pattern to ab21624, was used as a positive control.
Western blot - Nanog antibody - Embryonic Stem Cell Marker (ab21624)
All lanes : Anti-Nanog antibody - ChIP Grade (ab21624) at 1/1000 dilution
Lane 1 : whole cell extract of mouse 3T3 cells expressing mouse Nanog.
Lane 2 : whole cell extract of mouse 3T3 cells expressing human Nanog.
Predicted band size : 34 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
ab21624 recognizes human Nanog but does not recognize mouse Nanog in Western Blot.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nanog antibody - ChIP Grade (ab21624)
ab21624 staining Nanog in Human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/500 in PBS) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Protocols
References for Anti-Nanog antibody - ChIP Grade (ab21624)
This product has been referenced in:
- Hatano SYet al. Pluripotential competence of cells associated with Nanog activity. Mech Dev 122:67-79 (2005).Read more (PubMed: 15582778) »
- Yamaguchi Set al. Nanog expression in mouse germ cell development. Gene Expr Patterns 5:639-46 (2005).Read more (PubMed: 15939376) »
See all 18 publications for this product
Publishing research using ab21624? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"