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Our Abpromise guarantee covers the use of ab21624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/200. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
Some users report a stronger signal after blocking and diluting antibodies with 1% BSA instead of 5% milk.
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1E6C4] to Nanog (ab76586) as Capture.|
|ChIP||Use at an assay dependent concentration. PubMed: 18710938|
Immunocytochemical analysis of Human iPSCs labeling Nanog with ab21624 at 1/75 dilution.