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Does not react withMouse
Synthetic peptide: CGPEENYPSLQMSSAEMPHTE, corresponding to amino acids 29-49 of Human Nanog.
Our Abpromise guarantee covers the use of ab21624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
|WB||1/200. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
Some users report a stronger signal after blocking and diluting antibodies with 1% BSA instead of 5% milk.
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [1E6C4] to Nanog (ab76586). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1E6C4] to Nanog (ab76586) as Capture.|
|ChIP||Use at an assay dependent concentration. PubMed: 18710938|
ab21624 staining Nanog in Human H9 differentiated stem cells by Flow Cytometry. The sample was incubated with the primary antibody (1/100 in PBS + 5% FBS and 1% saponin) for 1 hour at 4°C. A FITC-conjugated Human anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"