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Synthetic peptide corresponding to Human Nanog aa 29-49.
Our Abpromise guarantee covers the use of ab21624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||1/200. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
Some users report a stronger signal after blocking and diluting antibodies with 1% BSA instead of 5% milk.
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1E6C4] to Nanog (ab76586) as Capture.|
|ChIP||Use at an assay dependent concentration. PubMed: 18710938|
ab21624 staining Nanog in human iCell® iPSC Diff Day 5 by Flow Cytometry. Cells were harvested in 1 mM EDTA/1 mM EGTA in PBS (calcium- and magnesium-free) and fixed with acetone. The sample was incubated with the primary antibody (1/200 in 10% FBS) for 2 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Gating Strategy: negative control cells.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"