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Anti-Nanog antibody - ChIP Grade (ab21624)

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Overview

Product name

Anti-Nanog antibody - ChIP Grade
See all Nanog products (22) ...

Description

Rabbit polyclonal to Nanog - ChIP Grade

Tested applications

IHC-P, ICC, Flow Cyt, ICC/IF, WB, Sandwich ELISA, ChIPmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse

Immunogen

Synthetic peptide: CGPEENYPSLQMSSAEMPHTE, corresponding to amino acids 29-49 of Human Nanog.

CGPEENYPSL QMSSAEMPHT E

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Sandwich ELISA - Nanog antibody - ChIP Grade (ab21624)Sandwich ELISA - Nanog antibody - ChIP Grade (ab21624) image (enlarge)

  • Immunofluorescence - Nanog antibody - Embryonic Stem Cell Marker (ab21624)Immunofluorescence - Nanog antibody - Embryonic Stem Cell Marker (ab21624) image (enlarge)

  • Western blot - Nanog antibody - Embryonic Stem Cell Marker (ab21624)Western blot - Nanog antibody - Embryonic Stem Cell Marker (ab21624) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab21624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

Tissue specificity

Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

Sequence similarities

Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain.

Developmental stage

Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ9H9S0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Embryonic stem cell specific homeobox protein (Nanog) antibody
  • ENK antibody
  • hNanog antibody
  • Homeobox protein NANOG antibody
  • Homeobox transcription factor Nanog antibody
  • NANOG antibody
  • Nanog homeobox antibody
  • NANOG_HUMAN antibody
see all

Anti-Nanog antibody - ChIP Grade images:

  Sandwich ELISA - Nanog antibody - ChIP Grade (ab21624)

Sandwich ELISA - Nanog antibody - ChIP Grade (ab21624)

Standard Curve for Nanog (Analyte: Nanog protein (ab50053)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [1E6C4] to Nanog (ab76586) at 0.2 ug/ml and Detector Antibody Rabbit polyclonal to Nanog - ChIP Grade (ab21624) at 0.5 ug/ml.

  Immunofluorescence - Nanog antibody - Embryonic Stem Cell Marker (ab21624)

Immunofluorescence - Nanog antibody - Embryonic Stem Cell Marker (ab21624)

ab21624 at a 1/1000 dilution staining Nanog in human embryonic stem cells by ICC. An antibody towards Oct4, which has an identical staining pattern to ab21624, was used as a positive control.

  Western blot - Nanog antibody - Embryonic Stem Cell Marker (ab21624)

Western blot - Nanog antibody - Embryonic Stem Cell Marker (ab21624)

All lanes : Anti-Nanog antibody - ChIP Grade (ab21624) at 1/1000 dilution

Lane 1 : whole cell extract of mouse 3T3 cells expressing mouse Nanog.
Lane 2 : whole cell extract of mouse 3T3 cells expressing human Nanog.


Predicted band size : 34 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)


ab21624 recognizes human Nanog but does not recognize mouse Nanog in Western Blot.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nanog antibody - ChIP Grade (ab21624)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nanog antibody - ChIP Grade (ab21624)

ab21624 staining Nanog in Human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/500 in PBS) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-Nanog antibody - ChIP Grade (ab21624)

This product has been referenced in:

See all 18 publications for this product

Publishing research using ab21624? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"