Products:Stem Cells >> Embryonic Stem Cells >> Intracellular
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab62734? Please let us know so that we can cite the reference in this datasheet
ab62734 has been referenced in 7 publications.
Publishing research using ab62734? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
3T3 mouse fibroblasts were transfected with a mammalian expression vector expressing human Nanog and stained with ab62734 (5 mg/mL) followed by Goat Anti-Mouse, Cy3 conjugate. A clear detection of Nanog can be seen in nuclei of transfected cells.
Lane 1 : Anti-Nanog antibody [NNG-811] (ab62734) at 10 µg/ml
Lane 2 : Anti-Nanog antibody [NNG-811] (ab62734) at 2 µg/ml
Lane 3 : Negative control – no primary antibody
Lane 1 : Cell extract of NT2 cells
Lane 2 : Cell extract of NT2 cells
Lane 3 : Cell extract of NT2 cells
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Mouse IgG
developed using the ECL technique
Predicted band size : 35 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
1
Call 01223 696 000 or contact us