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Products:Stem Cells >> Embryonic Stem Cells >> Intracellular
Anti-Nanog antibody [NNG-811] (ab62734)
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab62734 for help.
There are no answered questions for ab62734
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunocytochemistry/ Immunofluorescence - Nanog antibody [NNG-811] (ab62734)](/ps/datasheet/Images/62/ab62734/ab62734_IF.jpg)
Immunocytochemistry/ Immunofluorescence - Nanog antibody [NNG-811] (ab62734)
3T3 mouse fibroblasts were transfected with a mammalian expression vector expressing human Nanog and stained with ab62734 (5 mg/mL) followed by Goat Anti-Mouse, Cy3 conjugate. A clear detection of Nanog can be seen in nuclei of transfected cells.
![Western blot - Nanog antibody [NNG-811] (ab62734)](/ps/datasheet/Images/62/ab62734/ab62734_WB.jpg)
Western blot - Nanog antibody [NNG-811] (ab62734)
Lane 1 : Anti-Nanog antibody [NNG-811] (ab62734) at 10 µg/ml
Lane 2 : Anti-Nanog antibody [NNG-811] (ab62734) at 2 µg/ml
Lane 3 : Negative control – no primary antibody
Lane 1 : Cell extract of NT2 cells
Lane 2 : Cell extract of NT2 cells
Lane 3 : Cell extract of NT2 cells
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Mouse IgG
developed using the ECL technique
Predicted band size : 35 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
![Nanog antibody [NNG-811] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (62734)](/ps/datasheet/images/62/ab62734/Nanog-Primary-antibodies-ab62734-3.jpg)
Nanog antibody [NNG-811] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (62734)
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