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Anti-Nanog antibody [NNG-811] (ab62734)

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Overview

Product name

Anti-Nanog antibody [NNG-811]
See all Nanog products (22) ...

Description

Mouse monoclonal [NNG-811] to Nanog

Tested applications

WB, ICC/IF, IHC-P, IP, ELISAmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant full length human Nanog.

Positive control

Extracts of NT2 cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

NNG-811

Isotype

IgG1

Research areas

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Cancer >> Cell cycle >> Cell differentiation
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> SMADs
Stem Cells >> Embryonic Stem Cells >> Intracellular

  • Immunocytochemistry/ Immunofluorescence - Nanog antibody [NNG-811] (ab62734)Immunocytochemistry/ Immunofluorescence - Nanog antibody [NNG-811] (ab62734) image (enlarge)

  • Western blot - Nanog antibody [NNG-811] (ab62734)Western blot - Nanog antibody [NNG-811] (ab62734) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab62734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

Tissue specificity

Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

Sequence similarities

Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain.

Developmental stage

Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ9H9S0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Embryonic stem cell specific homeobox protein (Nanog) antibody
  • ENK antibody
  • FLJ12581 antibody
  • FLJ40451 antibody
  • hNanog antibody
  • Homeobox protein NANOG antibody
  • Homeobox transcription factor Nanog antibody
  • NANOG antibody
  • Nanog homeobox antibody
  • NANOG_HUMAN antibody
see all

Database links

Anti-Nanog antibody [NNG-811] images:

  Immunocytochemistry/ Immunofluorescence - Nanog antibody [NNG-811] (ab62734)

Immunocytochemistry/ Immunofluorescence - Nanog antibody [NNG-811] (ab62734)

3T3 mouse fibroblasts were transfected with a mammalian expression vector expressing human Nanog and stained with ab62734 (5 mg/mL) followed by Goat Anti-Mouse, Cy3 conjugate. A clear detection of Nanog can be seen in nuclei of transfected cells.

  Western blot - Nanog antibody [NNG-811] (ab62734)

Western blot - Nanog antibody [NNG-811] (ab62734)

Lane 1 : Anti-Nanog antibody [NNG-811] (ab62734) at 10 µg/ml
Lane 2 : Anti-Nanog antibody [NNG-811] (ab62734) at 2 µg/ml
Lane 3 : Negative control – no primary antibody

Lane 1 : Cell extract of NT2 cells
Lane 2 : Cell extract of NT2 cells
Lane 3 : Cell extract of NT2 cells

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Mouse IgG
developed using the ECL technique

Predicted band size : 35 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)

References for Anti-Nanog antibody [NNG-811] (ab62734)

This product has been referenced in:

  • Mah Net al. Molecular Insights into Reprogramming-Initiation Events Mediated by the OSKM Gene Regulatory Network. PLoS One 6:e24351 (2011). ICC/IF; Human.Read more (PubMed: 21909390) »
  • Prigione Aet al. Mitochondrial-Associated Cell Death Mechanisms Are Reset to an Embryonic-Like State in Aged Donor-Derived iPS Cells Harboring Chromosomal Aberrations. PLoS One 6:e27352 (2011). ICC/IF; Human.Read more (PubMed: 22110631) »

See all 7 publications for this product

Publishing research using ab62734? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"