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Proteins and Peptides
ab14960
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab14959? Please let us know so that we can cite the reference in this datasheet
ab14959 has been referenced in 2 publications.
- Ye S et al. Pleiotropy of Glycogen Synthase Kinase-3 Inhibition by CHIR99021 Promotes Self-Renewal of Embryonic Stem Cells from Refractory Mouse Strains. PLoS One 7:e35892 (2012). ICC/IF. PubMed: 22540008
- Fujita J et al. Caspase activity mediates the differentiation of embryonic stem cells. Cell Stem Cell 2:595-601 (2008). WB; Mouse. PubMed: 18522852
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
- Nanog antibody (ab14959)
Lanes 1-4 : Nanog antibody (ab14959) at 1 ug/ml
Lanes 3-4 : Nanog peptide (ab14960) at 1 ug/ml
Observed band size: 34.2 kDa
Additional bands at: 18 kDa (possible cross reactivity), 38 kDa (possible cross reactivity), 50 kDa (possible cross reactivity).
Lanes 1 and 3: Mouse embryonic stem cell lysate (positive control)
Lanes 2 and 4: Human embryonic stem cell lysate (negative control)
ab14959 recognised a band of the expected size for Nanog in mouse embryonic stem cells but not in human embryonic stem cells. Additional bands were also recognised in mouse embyonic stem cells.
Performed under reducing conditions.
Western blot - Nanog antibody (ab14959)
All lanes : Anti-Nanog antibody (ab14959) at 0.8 µg/ml
Lane 1 : ES Cell Nuclear Extract
Lane 2 : ES Cell Nuclear Extract
Predicted band size : 34 kDa
Adam Yates and Ian Chambers, University of Edinburgh
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody (ab14959)
ab14959 staining mouse teratoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab14959 at 1/600 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Carl Hobbs
Nanog antibody for WB in Mouse (14959)
Anti-Nanog antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (14959)
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