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Anti-Nanog antibody (ab14959)

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If your product does not perform as described on this datasheet, we will refund or replace your product...

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This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab14959 for help.

Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.

2 questions for ab14959

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Question 1

Monday 24-April-2006

Customer bought Nanog antibody and would like an isotype control. Staining on ES cells.

ANSWER:

  

Thank you for your enquiry.

There are at least three isotype controls you could use: normal rabbit serum (ab7487), ab27472, ab27478.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Question 2

Thursday 07-July-2005

What is the homology of your abs to bovine nanog (DQ069776)?

ANSWER:

  

Thank you for your enquiry and your patience in waiting for my reply.

I have compared the immunogen sequences with the sequence of bovine nanog using a ClustalW alignment. For ab14959 less than 30% of the sequence is homologous, for ab21624 the homology is 50%, and for ab21603 the epitope is not known but the overall sequence homology is approximately 50%. The end result in my opinion is that the homology is not high enough for any of these antibodies to cross-react with bovine nanog. You would want to see a higher degree of homology to have any confidence that these antibodies would cross-react with bovine nanog.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

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