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Products:Stem Cells >> Embryonic Stem Cells >> Intracellular
Overview
Product name
Anti-Nanog antibody
See all Nanog products (22) ...
Description
Rabbit polyclonal to Nanog
Tested applications
Cross reactivity
Reacts with
Mouse
Does not react with
Human
Immunogen
Synthetic peptide derived from residues 250 to the C-terminus of Mouse Nanog.
.
General notes
AF 21/11/07 - PH tested this for us. She can't see a band in WB using mouse ES cells - this is the opposite of the information provided by the lab and Ian Chambers that have managed to get a band of the correct size, so i have left the DS as is. PH also says it also doesn't work in IF.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). Can be blocked with Nanog peptide.
Is unsuitable for IF.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
Tissue specificity
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
Sequence similarities
Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain.
Developmental stage
Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9H9S0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- hNanog antibody
see all
Database links
- Entrez Gene: 71950 Mouse
- SwissProt: Q7TN85 Mouse
- SwissProt: Q80Z64 Mouse
- Unigene: 6047 Mouse
Anti-Nanog antibody images:
- Nanog antibody (ab14959)
Lanes 1-4 : Nanog antibody (ab14959) at 1 ug/ml
Lanes 3-4 : Nanog peptide (ab14960) at 1 ug/ml
Observed band size: 34.2 kDa
Additional bands at: 18 kDa (possible cross reactivity), 38 kDa (possible cross reactivity), 50 kDa (possible cross reactivity).
Lanes 1 and 3: Mouse embryonic stem cell lysate (positive control)
Lanes 2 and 4: Human embryonic stem cell lysate (negative control)
ab14959 recognised a band of the expected size for Nanog in mouse embryonic stem cells but not in human embryonic stem cells. Additional bands were also recognised in mouse embyonic stem cells.
Performed under reducing conditions.
Western blot - Nanog antibody (ab14959)
All lanes : Anti-Nanog antibody (ab14959) at 0.8 µg/ml
Lane 1 : ES Cell Nuclear Extract
Lane 2 : ES Cell Nuclear Extract
Predicted band size : 34 kDa
Adam Yates and Ian Chambers, University of Edinburgh
References for Anti-Nanog antibody (ab14959)
This product has been referenced in:
- Fujita Jet al. Caspase activity mediates the differentiation of embryonic stem cells. Cell Stem Cell 2:595-601 (2008). WB; Mouse.Read more (PubMed: 18522852) »
See 1 publication for this product
Publishing research using ab14959? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"