Anti-Nanog antibody (ab77095)

Overview

• Product nameAnti-Nanog antibodySee all Nanog primary antibodies ...
• Description
  Goat polyclonal to Nanog
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human, Pig
  Predicted to work with: Dog

  Does not react with

  Mouse
• Immunogen

  Synthetic peptide: QNQRMKSKRWQKNN by a Cysteine residue linker, corresponding to internal sequence amino acids 144-157 of Human Nanog

  Run BLAST with Run BLAST with
• Positive controlInduced pluriform stem cells derived from human keratinocytes. Human ovary lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 0.5% BSA, 99% Tris buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Developmental Biology
  • Embryogenesis
  • Embryonic stem cells
  • Surface molecules

  • Stem Cells
  • Embryonic Stem Cells
  • Intracellular

  • Cancer
  • Cell cycle
  • Cell differentiation

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • SMADs

Applications

Target

• FunctionTranscription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
• Tissue specificityExpressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
• Sequence similaritiesBelongs to the Nanog homeobox family.
  Contains 1 homeobox DNA-binding domain.
• Developmental stageExpressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q9H9S0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 100293888 Human
  • Entrez Gene: 79923 Human
  • Entrez Gene: 100170132 Pig
  • Omim: 607937 Human
  • SwissProt: Q9H9S0 Human
  • Unigene: 635882 Human

• Alternative names
  Embryonic stem cell specific homeobox protein (Nanog) antibodyENK antibodyFLJ12581 antibody

  FLJ40451 antibodyhNanog antibodyHomeobox protein NANOG antibodyHomeobox transcription factor Nanog antibodyNANOG antibodyNanog homeobox antibodyNANOG_HUMAN antibody

  see all

Anti-Nanog antibody images

• 

  Western blot - Nanog antibody (ab77095)
  Anti-Nanog antibody (ab77095) at 0.1 µg/ml + Human ovary lysate at 35 µg
  developed using the ECL technique
  
  Predicted band size : 35 kDa
  Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - Nanog antibody (ab77095)
  Immunofluorescence staining of a colony of induced pluriform stem cells derived from human keratinocytes labelled with ab77095 at 5µg/ml (green staining).