If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab80892? Please let us know so that we can cite the reference in this datasheet
ab80892 has been referenced in 6 publications.
Publishing research using ab80892? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab80892 staining Nanog in mouse embryonic stem cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in Tween 20 prior to blocking in 20% serum for 30 minutes at 25ºC. The primary antibody was diluted 1/100 and incubated with the sample for 4 hour at 25ºC. A Cy5 conjugated goat polyclonal to rabbit IgG antibody, diluted 1/100, was used as the secondary. Nuclear staining with DAPI.
This image is courtesy of an Abreview submitted by Vladimir Mazurov
IHC-Wholemount image of anti-Nanog antibody (ab80892) staining on a mouse pre-implantation embryo. This embryo was fixed in paraformaldehyde, permeabilized in 0.25% Triton X and then blocked in 5% serum for 1 hour at room temperature. It was stained with anti-Nanog antibody overnight at 4 degrees (right), and DAPI (left).
This image is courtesy of an anonymous abreview.
ab80892 staining mouse teratoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab80892 at 1/400 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Carl Hobbs
Overlay histogram of Nanog staining in mouse embryonic stem cells using ab80892, at 1:100 dilution. Purple histogram represents negative control (rabbit IgG); green line represents anti-Nanog antibody (ab80892).
This image is taken from an abreview by Kate Hawkins.
9
Call 01223 696 000 or contact us