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Products:Stem Cells >> Embryonic Stem Cells >> Intracellular
Overview
Product name
Anti-Nanog antibody
See all Nanog products (22) ...
Description
Rabbit polyclonal to Nanog
Tested applications
ICC, WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human, Monkey
Immunogen
Mouse Nanog protein
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Immunocytochemistry/ Immunofluorescence - Nanog antibody (ab80892)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab80892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC
ICC: 1/150 - 1/700.ICC: 1/150 - 1/700.
WB: 1/300 - 1/2000.Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IHC-P: 1/100((see Abreview))
ICC/IF: Use at an assay dependent dilution.
Target
Function
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
Tissue specificity
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
Sequence similarities
Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain.
Developmental stage
Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9H9S0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- hNanog antibody
see all
Database links
- Entrez Gene: 100293888 Human
- Entrez Gene: 79923 Human
- Entrez Gene: 71950 Mouse
- Omim: 607937 Human
- SwissProt: Q9H9S0 Human
- SwissProt: Q7TN85 Mouse
- SwissProt: Q80Z64 Mouse
- Unigene: 635882 Human
see all
Anti-Nanog antibody images:
Immunocytochemistry/ Immunofluorescence - Nanog antibody (ab80892)
ab80892 staining Nanog in mouse embryonic stem cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in Tween 20 prior to blocking in 20% serum for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 4 hour at 25°C. A Cy5 conjugated goat polyclonal to rabbit IgG antibody, diluted 1/100, was used as the secondary. Nuclear staining with DAPI.
This image is courtesy of an Abreview submitted by Vladimir Mazurov
References for Anti-Nanog antibody (ab80892)
This product has been referenced in:
- Czepiel Met al. Differentiation of induced pluripotent stem cells into functional oligodendrocytes. Glia 59:882-92 (2011).Read more (PubMed: 21438010) »
- Sustácková Get al. Differentiation-Independent Fluctuation of Pluripotency-Related Transcription Factors and Other Epigenetic Markers in Embryonic Stem Cell Colonies. Stem Cells Dev : (2011). ICC/IF; Mouse.Read more (PubMed: 21609209) »
See all 5 publications for this product
Publishing research using ab80892? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"