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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-Nanos Homologue 1 (NANOS1) antibody
See all Nanos Homologue 1 (NANOS1) products (5) ...
Description
Goat polyclonal to Nanos Homologue 1 (NANOS1)
Specificity
This antibody is expected to recognise the longer isoform 1 only, not isoform 2.
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: SARDGPPGKKLR, corresponding to amino acids 281-292 of Human Nanos Homologue 1 (NANOS1)
SARDGPPGKK LR
Positive control
Human testes lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
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Western blot - Nanos Homologue 1 (NANOS1) antibody (ab36163)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab36163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
IHC-P
Application notes
ELISA: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 2.5 µg/ml. (PMID 18223680)
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 30 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.
Tissue specificity
Testis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness.
Sequence similarities
Belongs to the nanos family.
Contains 1 nanos-type zinc finger.
Developmental stage
Fetal ovary and fetal testis (at protein level).
Domain
The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.
The N-terminal region and C-terminal zinc-finger RNA-binding domain are both necessary for interaction with SNAPIN.
Cellular localization
Cytoplasm > perinuclear region. Cytoplasm. Co-localizes with SNAPIN and PUM2 in the perinuclear region of germ cells.
Target information above from: UniProt accessionQ8WY41
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC Rep1a antibody
- EC_Rep1a antibody
- NANO1_HUMAN antibody
see all
Database links
- Entrez Gene: 340719 Human
- Omim: 608226 Human
- SwissProt: Q8WY41 Human
- Unigene: 591918 Human
Anti-Nanos Homologue 1 (NANOS1) antibody images:
Western blot - Nanos Homologue 1 (NANOS1) antibody (ab36163)
Anti-Nanos Homologue 1 (NANOS1) antibody (ab36163) at 0.3 µg/ml (incubated for 1 hour) + Human Testis lysate in RIPA buffer. at 35 µg
developed using the ECL technique
Predicted band size : 30 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
References for Anti-Nanos Homologue 1 (NANOS1) antibody (ab36163)
This product has been referenced in:
- Bonnomet Aet al. The E-cadherin-repressed hNanos1 gene induces tumor cell invasion by upregulating MT1-MMP expression. Oncogene 27:3692-9 (2008). IHC-P; Human.Read more (PubMed: 18223680) »
See 1 publication for this product
Publishing research using ab36163? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"