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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - Nanos Homologue 1 (NANOS1) antibody (ab65203)
Anti-Nanos Homologue 1 (NANOS1) antibody (ab65203) at 1/60 dilution + T47D cell line lysates at 35 µg
Predicted band size : 30 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nanos Homologue 1 (NANOS1) antibody (ab65203)
ab65203 at 1/50 dilution staining Nanos Homologue 1 in human breast carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
Immunocytochemistry/ Immunofluorescence - Nanos Homologue 1 (NANOS1) antibody (ab65203)
ab65203 at 1/50 dilution staining Nanos Homologue 1 in human Hela cells by Immunocytochemistry /Immunofluorescence. An Alexa-Fluor 546 conjugated donkey polyclonal to rabbit IgG was used as secondary.
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