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Western blot - Nanos Homologue 1 (NANOS1) antibody (ab65203)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nanos Homologue 1 (NANOS1) antibody (ab65203)
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Immunocytochemistry/ Immunofluorescence - Nanos Homologue 1 (NANOS1) antibody (ab65203)
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Product Name
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Nanos Homologue 1 (NANOS1) antibody
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See all Nanos Homologue 1 (NANOS1) antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Nanos Homologue 1 (NANOS1)
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Immunogen
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A KLH conjugated synthetic peptide selected from the C-terminal region of human Nanos Homologue 1 (NANOS1)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
ICC/IF: 1/10 - 1/50.
IHC-P: 1/10 - 1/50.
WB: 1/50 - 1/100. Detects a band of approximately 36 kDa (predicted molecular weight: 30 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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T47D cell line lysates.
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Cellular localization
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Cytoplasmic, perinuclear region
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Research areas
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Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
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Relevance
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NANOS1 may regulate the translation of specific mRNAs by forming a complex with PUM2 that associates with the 3' untranslated regions (UTRs) of mRNA targets. Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Ammonium Sulphate Precipitation
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Nanos Homologue 1 (NANOS1) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Nanos Homologue 1 (NANOS1) antibody images, references, products related to ab65203 and other tools.
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Nanos Homologue 1 (NANOS1) antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nanos Homologue 1 (NANOS1) antibody (ab65203)
ab65203 at 1/50 dilution staining Nanos Homologue 1 in human breast carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
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Immunocytochemistry/ Immunofluorescence - Nanos Homologue 1 (NANOS1) antibody (ab65203)
ab65203 at 1/50 dilution staining Nanos Homologue 1 in human Hela cells by Immunocytochemistry /Immunofluorescence. An Alexa-Fluor 546 conjugated donkey polyclonal to rabbit IgG was used as secondary.
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Search PubMed (MEDLINE) for references to Nanos Homologue 1 (NANOS1)
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Nanos Homologue 1 (NANOS1) antibody - more information
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Customer reviews (feedback) regarding Nanos Homologue 1 (NANOS1) antibody |
Customer FAQs regarding Nanos Homologue 1 (NANOS1) antibody |
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Nanos Homologue 1 (NANOS1) antibody - related products:
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