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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-Nanos Homologue 1 (NANOS1) antibody
See all Nanos Homologue 1 (NANOS1) products (5) ...
Description
Rabbit polyclonal to Nanos Homologue 1 (NANOS1)
Tested applications
ICC/IF, IHC-P, WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
A KLH conjugated synthetic peptide selected from the C-terminal region of human Nanos Homologue 1 (NANOS1)
Positive control
T47D cell line lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/10 - 1/50.
IHC-P: 1/10 - 1/50.
WB: 1/50 - 1/100.Detects a band of approximately 36 kDa (predicted molecular weight: 30 kDa).
ELISA
ELISA: 1/1000ELISA: 1/1000
Target
Function
May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.
Tissue specificity
Testis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness.
Sequence similarities
Belongs to the nanos family.
Contains 1 nanos-type zinc finger.
Developmental stage
Fetal ovary and fetal testis (at protein level).
Domain
The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.
The N-terminal region and C-terminal zinc-finger RNA-binding domain are both necessary for interaction with SNAPIN.
Cellular localization
Cytoplasm > perinuclear region. Cytoplasm. Co-localizes with SNAPIN and PUM2 in the perinuclear region of germ cells.
Target information above from: UniProt accessionQ8WY41
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC Rep1a antibody
- EC_Rep1a antibody
- NANO1_HUMAN antibody
see all
Database links
- Entrez Gene: 340719 Human
- Omim: 608226 Human
- SwissProt: Q8WY41 Human
- Unigene: 591918 Human
Anti-Nanos Homologue 1 (NANOS1) antibody images:
Western blot - Nanos Homologue 1 (NANOS1) antibody (ab65203)
Anti-Nanos Homologue 1 (NANOS1) antibody (ab65203) at 1/60 dilution + T47D cell line lysates at 35 µg
Predicted band size : 30 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nanos Homologue 1 (NANOS1) antibody (ab65203)
ab65203 at 1/50 dilution staining Nanos Homologue 1 in human breast carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
Immunocytochemistry/ Immunofluorescence - Nanos Homologue 1 (NANOS1) antibody (ab65203)
ab65203 at 1/50 dilution staining Nanos Homologue 1 in human Hela cells by Immunocytochemistry /Immunofluorescence. An Alexa-Fluor 546 conjugated donkey polyclonal to rabbit IgG was used as secondary.
References for Anti-Nanos Homologue 1 (NANOS1) antibody (ab65203)
ab65203 has not yet been referenced specifically in any publications.
Publishing research using ab65203? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"