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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab70001? Please let us know so that we can cite the reference in this datasheet
ab70001 has been referenced in 1 publications.
- Julaton VT & Reijo Pera RA NANOS3 function in human germ cell development. Hum Mol Genet 20:2238-50 (2011). WB; Human. PubMed: 21421998
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Nanos3 antibody (ab70001)
Anti-Nanos3 antibody (ab70001) at 2 µg/ml + Human brain tissue lysate at 15 µg
Predicted band size : 19 kDa
Observed band size : 19 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nanos3 antibody (ab70001)
ab70001 at 2.5µg/ml staining Nanos3 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
Immunocytochemistry/ Immunofluorescence - Anti-Nanos3 antibody (ab70001)
ICC/IF image of ab70001 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70001, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-Nanos3 antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Rhesus monkey (70001)
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