Anti-Nanos3 antibody (ab70001)
Key features and details
- Rabbit polyclonal to Nanos3
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Nanos3 antibody -
Description
Rabbit polyclonal to Nanos3 -
Host species
Rabbit -
Specificity
ab70001 does not cross-react with either Nanos1 or Nanos2. -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic 16 amino acid peptide near the carboxy terminus of the human Nanos3 (GenBank accession no. P60323).
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Positive control
- IHC-P: Human brain tissue. WB: Human brain tissue lysate. ICC/IF: Human brain cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use a concentration of 2.5 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 2.5 µg/ml. |
Target
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Function
Plays a role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Affects cell proliferation probably by repressing translation of specific mRNAs. Maintains the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways. Essential in the early stage embryo to protect the migrating primordial germ cells (PGCs) from apoptosis. -
Tissue specificity
Ovary, testis and brain (at protein level). In the ovaries, expressed during multiple stages of oogenesis, including primordial, primary, secondary and antral follicles with the highest expression in the oocytes. In the testis, expressed in germ cells, type A spermatogonia (SA), primary spermatocytes (S1), round spermatids (S3) and elongated spermatids. -
Sequence similarities
Belongs to the nanos family.
Contains 1 nanos-type zinc finger. -
Developmental stage
Fetal ovary and fetal testis (at protein level). -
Domain
The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein. -
Cellular localization
Nucleus. Cytoplasm. Cytoplasmic granule. Cytoplasm > P-body. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with PUM2, EIF2S1 and TIAL1 in the stress granules. Co-localizes with DCP1A in the P-body. - Information by UniProt
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Database links
- Entrez Gene: 342977 Human
- Entrez Gene: 244551 Mouse
- Entrez Gene: 288909 Rat
- Omim: 608229 Human
- SwissProt: P60323 Human
- SwissProt: P60324 Mouse
- Unigene: 127982 Human
- Unigene: 216740 Mouse
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Alternative names
- NANO3_HUMAN antibody
- Nanos homolog 3 antibody
- NANOS3 antibody
see all
Images
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ab70001 at 2.5µg/ml staining Nanos3 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
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Anti-Nanos3 antibody (ab70001) at 2 µg/ml + Human brain tissue lysate at 15 µg
Predicted band size: 19 kDa
Observed band size: 19 kDa -
Immunofluorescence of Nanos3 in Human Brain cells using ab70001 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab70001 has been referenced in 12 publications.
- Ma Z et al. Activation of embryonic/germ cell-like axis links poor outcomes of gliomas. Cancer Cell Int 22:371 (2022). PubMed: 36435765
- Cho J et al. Vascular remodeling by placenta-derived mesenchymal stem cells restores ovarian function in ovariectomized rat model via the VEGF pathway. Lab Invest 101:304-317 (2021). PubMed: 33303971
- Wang M et al. Deciphering the autophagy regulatory network via single-cell transcriptome analysis reveals a requirement for autophagy homeostasis in spermatogenesis. Theranostics 11:5010-5027 (2021). PubMed: 33754041
- Zhang F et al. Nanos3, a cancer-germline gene, promotes cell proliferation, migration, chemoresistance, and invasion of human glioblastoma. Cancer Cell Int 20:197 (2020). WB . PubMed: 32508533
- Zhao J et al. Expression pattern and potential role of Nanos3 in regulating testosterone biosynthesis in Leydig cells of sheep. Theriogenology 154:31-42 (2020). PubMed: 32474303