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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-Nanos3 antibody
Description
Rabbit polyclonal to Nanos3
Specificity
ab70001 does not cross-react with either Nanos1 or Nanos2.
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic 16 amino acid peptide near the carboxy terminus of the human Nanos3 (GenBank accession no. P60323).
Positive control
Human brain tissue lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Reproduction >> Germ cell markers
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Cell Biology >> Cell Cycle >> Cell differentiation
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
IHC-P: Use a concentration of 2.5 µg/ml
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
Plays a role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Affects cell proliferation probably by repressing translation of specific mRNAs. Maintains the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways. Essential in the early stage embryo to protect the migrating primordial germ cells (PGCs) from apoptosis.
Tissue specificity
Ovary, testis and brain (at protein level). In the ovaries, expressed during multiple stages of oogenesis, including primordial, primary, secondary and antral follicles with the highest expression in the oocytes. In the testis, expressed in germ cells, type A spermatogonia (SA), primary spermatocytes (S1), round spermatids (S3) and elongated spermatids.
Sequence similarities
Belongs to the nanos family.
Contains 1 nanos-type zinc finger.
Developmental stage
Fetal ovary and fetal testis (at protein level).
Domain
The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.
Cellular localization
Nucleus. Cytoplasm. Cytoplasmic granule. Cytoplasm > P-body. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with PUM2, EIF2S1 and TIAL1 in the stress granules. Co-localizes with DCP1A in the P-body.
Target information above from: UniProt accessionP60323
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- NANO3_HUMAN antibody
- Nanos homolog 3 antibody
- NANOS3 antibody
see all
Database links
- Entrez Gene: 342977 Human
- Entrez Gene: 244551 Mouse
- Entrez Gene: 288909 Rat
- Omim: 608229 Human
- SwissProt: P60323 Human
- SwissProt: Q495E5 Human
- SwissProt: P60324 Mouse
- Unigene: 127982 Human
see all
Anti-Nanos3 antibody images:
Western blot - Nanos3 antibody (ab70001)
Anti-Nanos3 antibody (ab70001) at 2 µg/ml + Human brain tissue lysate at 15 µg
Predicted band size : 19 kDa
Observed band size : 19 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nanos3 antibody (ab70001)
ab70001 at 2.5µg/ml staining Nanos3 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
Immunocytochemistry/ Immunofluorescence - Anti-Nanos3 antibody (ab70001)
ICC/IF image of ab70001 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70001, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Nanos3 antibody (ab70001)
This product has been referenced in:
- Julaton VT & Reijo Pera RA NANOS3 function in human germ cell development. Hum Mol Genet 20:2238-50 (2011). WB; Human.Read more (PubMed: 21421998) »
See 1 publication for this product
Publishing research using ab70001? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"