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Nardilysin protein (Tagged-His Tag) (ab92081)

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Overview

  • Product nameNardilysin protein (Tagged-His Tag)See all Nardilysin proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 958-1148 (191 amino acids) of Human Nardilysin (Isoform b) with an N terminal His tag. Predicted mwt: 23 kDa; NCBI locus NP_001095132.1.
  • Expression hostE. coli

    • Properties

  • Purification notes Purity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 92 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesLGYHVYPTCRNTSGILGFSVTVGTQATKYNSEVVDKKIEE FLSSFEEKIENLTEEAFNTQ VTALIKLKECEDTHLGEE VDRNWNEVVTQQYLFDRLAHEIEALKSFSKSDLVNWFKAH RG PGSKMLSVHVVGYGKYELEEDGTPSSEDSNSSCEVM QLTYLPTSPLLADCIIPITDIRAF TTTLNLLPYHK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92081 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        EC 3.4.24.61hNRD1hNRD2
        N arginine dibasic convertaseNardilysin precursorNRD CNRD convertaseNRD1
      see all
  • RelevanceA member of the metalloproteinase family M16, nardilysin is most similar to insulin degrading enzyme, and the bacterial peptidase pitrilysin. It cleaves peptide substrates on the N terminus of arginine residues in dibasic pairs. Nardilysin was first described as a processing enzyme of somatostatin 1-28, which is cleaved at the Arg-Lys paired amino acid location. Nardilysin was named N arginine dibasic convertase (NRD convertase) to reflect this cleavage, although it was later shown that the activity was due to aminopeptidase B acting as a heterodimer with nardilysin. In addition to somatostatin 1-28, nardilysin cleaves dynorphin A and a-neoendorphin. Nardilysin was first detected in the brain and testis, and later in the heart, skeletal muscle, and in lesser amounts in most tissues and cell lines.

    • Nardilysin protein (Tagged-His Tag) images

    • SDS-PAGE - Nardilysin protein (Tagged-His Tag) (ab92081)
      SDS-PAGE - Nardilysin protein (Tagged-His Tag) (ab92081)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Nardilysin protein (Tagged-His Tag) (ab92081)

    ab92081 has not yet been referenced specifically in any publications.

    Publishing research using ab92081 ? Please let us know so that we can cite the reference in this datasheet

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