Native Chicken Deglycosylated Avidin protein (HRP) (ab136212)
Key features and details
- Expression system: Native
- Purity: > 90% SDS-PAGE
- Suitable for: ELISA, Flow Cyt, WB
Description
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Product name
Native Chicken Deglycosylated Avidin protein (HRP)
See all Deglycosylated Avidin proteins and peptides -
Biological activity
ab136212 reacts with D-Biotin. -
Purity
> 90 % SDS-PAGE.
Purification methods are proprietary, using a combination of precipitation and/or chromatography techniques. Purification percentages provided relate prior to conjugation. -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Chicken -
Predicted molecular weight
60 kDa -
Amino acids
1 to 152
-
-
Conjugation
HRP
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab136212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
Flow Cytometry
Western blot
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Form
Liquid -
Additional notes
Deglycosylated acidic avidin has higher affinity for biotin than standard avidin and therefore reduces background staining. ab136212 reacts with D-Biotin. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Store In the Dark.
pH: 7.4
Constituents: 50% PBS, 49% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- AVD
- AVID_CHICK
- Avidin
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Function
The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin). -
Tissue specificity
Synthesized in hen oviduct and concentrated in egg white (where it represent 0.05% of the total protein). -
Sequence similarities
Belongs to the avidin/streptavidin family.
Contains 1 avidin-like domain. -
Post-translational
modificationsN-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab136212 has not yet been referenced specifically in any publications.