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Inquiry: Dear Sir/Madam We have purchased abcam Protein A HRP(ab 7456) My query is regarding the dilution of Protein A HRP(ab 7456) to be used in western blotting for development with DAB as substrate
Thanking you Sincerely
Asked on Aug 08 2012
Thank you for your enquiry.
The best dilution of ab7456 for your application will depend on thethe individual experiment which will require some individual optimization. However, I can suggest dilutions of from 1:1000 and 1:5000 are usually good starting points for optimization .
I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.
Abcam Scientific Support
Answered on Aug 08 2012