Native human Cathepsin G protein (ab91122)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Native human Cathepsin G protein
See all Cathepsin G proteins and peptides -
Biological activity
Activity: 2-4 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one µmole of Suc-Ala-Ala Pro-Phe-pNA (1 mM) per minute at 25°C in 160 mM Tris-HCl, pH 7.4, with 1.6 M NaCl. -
Purity
> 95 % SDS-PAGE. -
Expression system
Native -
Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab91122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Additional notes
Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 0.66. Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti- HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20ºC.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionCentrifuge the vial prior to opening. Reconstitute in 150 mM sodium acetate (pH 5.5), 150 mM NaCl.
General Info
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Alternative names
- CATG
- CATG_HUMAN
- Cathepsin G
see all -
Function
Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Cellular localization
Cell surface. - Information by UniProt
Images
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SDS-PAGE: 12% Tris-HCl gel
Lane 1. 5 μg Cathepsin G (reduced/heated)
Lane 2. 10 μg Cathepsin G (reduced/heated)
Lane 3. 20 μg Cathepsin G (reduced/heated)
Lane 4. Molecular weight markers
Lane 5. 5 μg Cathepsin G (non-reduced/no heat)
Lane 6. 10 μg Cathepsin G (non-reduced/no heat)
Lane 7. 20 μg Cathepsin G (non-reduced/no heat)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab91122 has been referenced in 1 publication.
- Kao SS et al. The effect of neutrophil serine proteases on human nasal epithelial cell barrier function. Int Forum Allergy Rhinol 9:1220-1226 (2019). PubMed: 31403754