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Anti-Nav1.5 antibody (ab56240) at 0.25 µg/ml + SW620 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 227 kDa
Observed band size : 168 kDa (why is the actual band size different from the predicted?)
Gel concentration: 8%
ab56240 (4µg/ml) staining Human Nav1.5 in skin tissue by immunohistochemistry on paraffin embedded tissue. The arrows indicate positively stained squamous epithelial cells. Magnification 400X.
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