Anti-Nav1.6/SCN8A antibody (ab65166)
Key features and details
- Rabbit polyclonal to Nav1.6/SCN8A
- Suitable for: IHC-Fr, IHC-P, IHC-FrFl, IHC-FoFr, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Nav1.6/SCN8A antibody
See all Nav1.6/SCN8A primary antibodies -
Description
Rabbit polyclonal to Nav1.6/SCN8A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, IHC-P, IHC-FrFl, IHC-FoFr, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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IHC-FrFl | (1) |
Use at an assay dependent concentration.
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 27905510
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ICC/IF | (1) |
Use at an assay dependent concentration.
See Abreview. |
WB | (1) |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-FrFl
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 27905510 |
ICC/IF
Use at an assay dependent concentration. See Abreview. |
WB
Use at an assay dependent concentration. |
Target
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Function
Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. In macrophages and melanoma cells, isoform 5 may participate in the control of podosome and invadopodia formation. -
Tissue specificity
Isoform 5 is expressed in non-neuronal tissues, such as monocytes/macrophages. -
Sequence similarities
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.6/SCN8A subfamily.
Contains 1 IQ domain. -
Domain
The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position. -
Post-translational
modificationsMay be ubiquitinated by NEDD4L; which would promote its endocytosis. -
Cellular localization
Membrane and Cytoplasmic vesicle. Some vesicles are localized adjacent to melanoma invadopodia and macrophage podosomes. Does not localize to the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 6334 Human
- Entrez Gene: 20273 Mouse
- Entrez Gene: 29710 Rat
- Omim: 600702 Human
- SwissProt: Q9UQD0 Human
- SwissProt: Q9WTU3 Mouse
- SwissProt: O88420 Rat
- Unigene: 710638 Human
see all -
Alternative names
- CerIII antibody
- CIAT antibody
- EIEE13 antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (12)
ab65166 has been referenced in 12 publications.
- Yang X et al. Social Deficits and Cerebellar Degeneration in Purkinje Cell Scn8a Knockout Mice. Front Mol Neurosci 15:822129 (2022). PubMed: 35557557
- Liu W et al. Altered expression of voltage gated sodium channel Nav1.1 is involved in motor ability in MPTP-treated mice. Brain Res Bull 170:187-198 (2021). PubMed: 33610724
- Okada T et al. Pain induces stable, active microcircuits in the somatosensory cortex that provide a therapeutic target. Sci Adv 7:N/A (2021). PubMed: 33741588
- Montero MC et al. Neosaxitoxin Inhibits the Expression of Inflammation Markers of the M1 Phenotype in Macrophages. Mar Drugs 18:N/A (2020). PubMed: 32471037
- Tan YX et al. Exercise-Induced Cognitive Improvement Is Associated with Sodium Channel-Mediated Excitability in APP/PS1 Mice. Neural Plast 2020:9132720 (2020). PubMed: 32256560