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Product Name
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Nck antibody [NC-20 (same as 20B-1H9)]
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See all Nck antibodies (8)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [NC-20 (same as 20B-1H9)] to Nck
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Immunogen
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Recombinant full length protein (Human).
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Immunoprecipitation (native and denatured): use at a concentration of 2µg / mg.
Western Blotting: use at a concentration of 2 - 4µg / ml for 2hrs at RT.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
By Western blot, this antibody detects a band of 47 kDa, which corresponds to the predicted molecular weight of Nck.
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Positive control
(see definition)
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A431 cells.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Signal transduction >> Other
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
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Relevance
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Nck is a highly conserved, oncogenic protein. It is a common target for the action of different surface receptors, encoding one SH2 and three SH3 domains, the Src homology motifs found in nonreceptor tyrosine kinases, Ras GTPase activating protein, phosphatidylinositol 3 kinase, and phospholipase Cg. Nck is widely expressed in various tissues and in cell lines from human, murine, and rat origins. Nck is phosphorylated on tyrosine, serine, and threonine residues in response to stimulation of EGF and PDGF in A431 and NIH 3T3 cells respectively. Like other SH2 containing proteins, Nck is associated with tyrosine autophosphorylated EGF or PDGF receptors via its SH2 domain. Overexpression of Nck leads to transformation of NIH 3T3 cells.
Nck is a highly conserved protein that has one SH2 and three SH3 domains. It is ubiquitously expressed in mammalian tissues. Nck is phosphorylated on tyrosine, serine and threonine residues in response to stimulation by EGF and PDGF. Nck has been proposed to function as an adaptor protein by coupling protein tyrosine kinases to signaling pathways that lead to cell proliferation. Over-expression of Nck results in transformation of NIH 3T3 cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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NC-20 (same as 20B-1H9)
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Nck antibody [NC-20 (same as 20B-1H9)] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Nck
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Nck antibody [NC-20 (same as 20B-1H9)] - more information
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