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Anti-Nck antibody [Y531] (ab32120)

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Overview

Product name

Anti-Nck antibody [Y531]
See all Nck products (8) ...

Description

Rabbit monoclonal [Y531] to Nck

Specificity

ab32120 recognises Nck.

Tested applications

WB, IHC-P, ICC, Flow Cyt, IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide corresponding to residues close to the SH2 domain of human Nck

Positive control

Hela cell lysate and human breast carcinoma

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.5% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

Y531

Isotype

IgG

  • Western blot - Nck antibody [Y531] (ab32120)Western blot - Nck antibody [Y531] (ab32120) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - Nck antibody [Y531] (ab32120)Immunohistochemistry (Paraffin-embedded sections) - Nck antibody [Y531] (ab32120) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/2000 - 1/10000.Det...Read more →

    WB: 1/2000 - 1/10000.Detects a band of approximately 47 kDa.

  • 1 Image

    IHC-P

     IHC-P: 1/100 - 1/250.

    IHC-P: 1/100 - 1/250.

  • ICC

     ICC: 1/250 - 1/500.

    ICC: 1/250 - 1/500.

  • Flow Cyt

     Flow Cyt: 1/60

    Flow Cyt: 1/60

  • IP

     IP: 1/50

    IP: 1/50

Target

Function

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.

Sequence similarities

Contains 1 SH2 domain.
Contains 3 SH3 domains.

Post-translational
modifications

Phosphorylated on Ser and Tyr residues.

Cellular localization

Cytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.

Target information above from: UniProt accessionP16333 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Cytoplasmic protein NCK1 antibody
  • Melanoma Nck protein antibody
  • MGC12668 antibody
  • NCK 1 antibody
  • Nck adaptor protein 1 antibody
  • NCK alpha antibody
  • NCK antibody
  • NCK tyrosine kinase antibody
  • Nck-1 antibody
  • NCK1 antibody
  • NCK1_HUMAN antibody
  • NCKalpha antibody
  • Non catalytic region of tyrosine kinase antibody
  • SH2/SH3 adaptor protein NCK alpha antibody
  • SH2/SH3 adaptor protein NCK-alpha antibody
  • SH2/SH3 adaptor protein NCKalpha antibody
see all

Anti-Nck antibody [Y531] images:

  Western blot - Nck antibody [Y531] (ab32120)

Western blot - Nck antibody [Y531] (ab32120)

Anti-Nck antibody [Y531] (ab32120) at 1/10000 dilution + Hela cell lysate

Observed band size : 47 kDa (why is the actual band size different from the predicted?)
Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

  Immunohistochemistry (Paraffin-embedded sections) - Nck antibody [Y531] (ab32120)

Immunohistochemistry (Paraffin-embedded sections) - Nck antibody [Y531] (ab32120)

ab32120, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue

References for Anti-Nck antibody [Y531] (ab32120)

ab32120 has not yet been referenced specifically in any publications.

Publishing research using ab32120? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"