Nck antibody [Y531] (ab32120)

Product Name 

Nck antibody [Y531]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [Y531] to Nck

Immunogen 

A synthetic peptide corresponding to residues close to the SH2 domain of human Nck

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab32120 recognises Nck.

Tested applications 
(see key)

Flow Cyt, ICC, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/60.
ICC: 1/250 - 1/500.
IHC-P: 1/100 - 1/250.
IP: 1/50.
WB: 1/2000 - 1/10000. Detects a band of approximately 47 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Hela cell lysate and human breast carcinoma

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Signal transduction >> Other
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly

Relevance 

Nck is a highly conserved, oncogenic protein. It is a common target for the action of different surface receptors, encoding one SH2 and three SH3 domains, the Src homology motifs found in nonreceptor tyrosine kinases, Ras GTPase activating protein, phosphatidylinositol 3 kinase, and phospholipase Cg. Nck is widely expressed in various tissues and in cell lines from human, murine, and rat origins. Nck is phosphorylated on tyrosine, serine, and threonine residues in response to stimulation of EGF and PDGF in A431 and NIH 3T3 cells respectively. Like other SH2 containing proteins, Nck is associated with tyrosine autophosphorylated EGF or PDGF receptors via its SH2 domain. Overexpression of Nck leads to transformation of NIH 3T3 cells.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

Y531

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.5% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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