![Western blot - Nck antibody [Y531] (ab32120) image](#ab32120-1.JPG)
![Immunohistochemistry (Paraffin-embedded sections) - Nck antibody [Y531] (ab32120) image](#ab32120-2.JPG)
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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Overview
Product name
Anti-Nck antibody [Y531]
See all Nck products (8) ...
Description
Rabbit monoclonal [Y531] to Nck
Specificity
ab32120 recognises Nck.
Tested applications
WB, IHC-P, ICC, Flow Cyt, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues close to the SH2 domain of human Nck
Positive control
Hela cell lysate and human breast carcinoma
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.5% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
Y531
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Signal transduction >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000.Detects a band of approximately 47 kDa.
IHC-P: 1/100 - 1/250.
ICC
ICC: 1/250 - 1/500.ICC: 1/250 - 1/500.
Flow Cyt
Flow Cyt: 1/60Flow Cyt: 1/60
IP
IP: 1/50IP: 1/50
Target
Function
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.
Sequence similarities
Contains 1 SH2 domain.
Contains 3 SH3 domains.
Post-translational
modifications
Phosphorylated on Ser and Tyr residues.
Cellular localization
Cytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
Target information above from: UniProt accessionP16333
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cytoplasmic protein NCK1 antibody
- Melanoma Nck protein antibody
- MGC12668 antibody
see all
Database links
- Entrez Gene: 4690 Human
- Entrez Gene: 17973 Mouse
- Entrez Gene: 300955 Rat
- Omim: 600508 Human
- SwissProt: P16333 Human
- SwissProt: Q99M51 Mouse
- SwissProt: B2RZ33 Rat
- Unigene: 477693 Human
Anti-Nck antibody [Y531] images:
Western blot - Nck antibody [Y531] (ab32120)
![Western blot - Nck antibody [Y531] (ab32120)](/ps/datasheet/Images/32/ab32120/ab32120-1.JPG)
Anti-Nck antibody [Y531] (ab32120) at 1/10000 dilution + Hela cell lysate
Observed band size : 47 kDa (why is the actual band size different from the predicted?)
Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - Nck antibody [Y531] (ab32120)
![Immunohistochemistry (Paraffin-embedded sections) - Nck antibody [Y531] (ab32120)](/ps/datasheet/Images/32/ab32120/ab32120-2.JPG)
ab32120, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue
References for Anti-Nck antibody [Y531] (ab32120)
ab32120 has not yet been referenced specifically in any publications.
Publishing research using ab32120? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"