Feedback Form

Western blot - Nck antibody [Y531] (ab32120)
Western blot - Nck antibody [Y531] (ab32120)
(enlarge)


Immunohistochemistry (Paraffin-embedded sections) - Nck antibody [Y531] (ab32120)
Immunohistochemistry (Paraffin-embedded sections) - Nck antibody [Y531] (ab32120)
(enlarge)


Product Name 

Nck antibody [Y531]

 

See all Nck antibodies (8)...

Product type 

Primary antibodies

Description 

Rabbit monoclonal [Y531] to Nck

Immunogen 

A synthetic peptide corresponding to residues close to the SH2 domain of human Nck

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab32120 recognises Nck.

Tested applications 
(see key)

Flow Cyt, ICC, IHC-P, IP, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews

Submit an Abreview

Be the first to review this antibody and earn 220 Abpoints.


Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/60.
ICC: 1/250 - 1/500.
IHC-P: 1/100 - 1/250.
IP: 1/50.
WB: 1/2000 - 1/10000. Detects a band of approximately 47 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Hela cell lysate and human breast carcinoma

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Signal transduction >> Other
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly

Relevance 

Nck is a highly conserved, oncogenic protein. It is a common target for the action of different surface receptors, encoding one SH2 and three SH3 domains, the Src homology motifs found in nonreceptor tyrosine kinases, Ras GTPase activating protein, phosphatidylinositol 3 kinase, and phospholipase Cg. Nck is widely expressed in various tissues and in cell lines from human, murine, and rat origins. Nck is phosphorylated on tyrosine, serine, and threonine residues in response to stimulation of EGF and PDGF in A431 and NIH 3T3 cells respectively. Like other SH2 containing proteins, Nck is associated with tyrosine autophosphorylated EGF or PDGF receptors via its SH2 domain. Overexpression of Nck leads to transformation of NIH 3T3 cells.

 

Alternative names Nck antibody [Y531] (ab32120)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

4690    (Human)
17973    (Mouse)
300955    (Rat)

Omim

    

600508    (Human)

SwissProt

    

P16333    (Human)
Q99M51    (Mouse)
B2RZ33    (Rat)

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

Y531

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.5% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Nck antibody [Y531] is on this datasheet. But please do contact us if you would like any reassurance!


See below for Nck antibody [Y531] images, references, products related to ab32120 and other tools.

 

Nck antibody [Y531] images:

Product image  Western blot - Nck antibody [Y531] (ab32120)

Western blot - Nck antibody [Y531] (ab32120)

Nck antibody [Y531] (ab32120) at 1/10000 dilution + Hela cell lysate

Observed band size : 47 kDa (why is the actual band size different from the predicted?)
Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

 

Product image  Immunohistochemistry (Paraffin-embedded sections) - Nck antibody [Y531] (ab32120)

Immunohistochemistry (Paraffin-embedded sections) - Nck antibody [Y531] (ab32120)

ab32120, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue

 
 

Search PubMed (MEDLINE) for references to Nck

 

Nck antibody [Y531] - more information

Customer reviews (feedback) regarding Nck antibody [Y531]

Customer FAQs regarding Nck antibody [Y531]

Protocols for Nck antibody [Y531]

Price and availability of products related to Nck antibody [Y531]

 

Nck antibody [Y531] - related products:

Products for Immunocytochemistry

Subcellular Markers

Products for Western blot

Loading controls

Lysates

 

Positive Controls for Nck antibody [Y531]

ab14655

  

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

ab4700

  

Breast tumor (human): medullary carcinoma tissue slides

 

Compatible Secondaries for Nck antibody [Y531]

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other Nck Antibodies

See all Nck antibodies (8)...

 

Nck antibody [Y531] - other tools:

Contact Abcam with a Technical Enquiry about Nck antibody [Y531]

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.