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Product Name
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Nck antibody
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See all Nck antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Nck
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Immunogen
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Fusion protein: MAEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKSWWR VRNSMNKTGF VPSNYVERKN SARKASIVKN LKDTLGIGKV KRKPSVPDSA SPADDSFVDP GERLYDLNMP AYVKFNYMAE REDELSLIKG TKVIVMEKCS DGWWRGSYNG QVGWFPSNYV TEEGDSPLGD HVGSLSEKLA AVVNNLNTGQ VLHVVQALYP FSSSNDEELN FEKGDVMDVI EKPENDPEWW KCRKINGMVG LVPKNYVTVM QNNP, corresponding to amino acids 1-254 of Human Nck produced in E. coli.
BLAST 'MAEEVVVVAK FDYVAQQEQ E LDIKKNER LW LLDDSKS WWR VRNSMN KTGF VPSNY VERKN SARK ASIVKN LKD TLGIGKV KR KPSVPDSA S PADDSFVDP GERLYDLNMP AYVKFNYMA E REDELSLI KG TKVIVME KCS DGWWRG SYNG QVGWF PSNYV TEEG DSPLGD HVG SLSEKLA AV VNNLNTGQ V LHVVQALYP FSSSNDEELN FEKGDVMDV I EKPENDPE WW KCRKING MVG LVPKNY ' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at an assay dependent dilution. Detects a band of approximately 42 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Non stimulated A431 kidney cell lysate or NIH3T3 cells.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Signal transduction >> Other
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
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Relevance
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Nck is a highly conserved, oncogenic protein. It is a common target for the action of different surface receptors, encoding one SH2 and three SH3 domains, the Src homology motifs found in nonreceptor tyrosine kinases, Ras GTPase activating protein, phosphatidylinositol 3 kinase, and phospholipase Cg. Nck is widely expressed in various tissues and in cell lines from human, murine, and rat origins. Nck is phosphorylated on tyrosine, serine, and threonine residues in response to stimulation of EGF and PDGF in A431 and NIH 3T3 cells respectively. Like other SH2 containing proteins, Nck is associated with tyrosine autophosphorylated EGF or PDGF receptors via its SH2 domain. Overexpression of Nck leads to transformation of NIH 3T3 cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: 0.1M Tris-glycine. pH 7.0
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Nck antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Nck
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Nck antibody - more information
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