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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Overview
Product name
Anti-Nck antibody
See all Nck products (8) ...
Description
Rabbit polyclonal to Nck
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Fusion protein: MAEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKSWWR VRNSMNKTGF VPSNYVERKN SARKASIVKN LKDTLGIGKV KRKPSVPDSA SPADDSFVDP GERLYDLNMP AYVKFNYMAE REDELSLIKG TKVIVMEKCS DGWWRGSYNG QVGWFPSNYV TEEGDSPLGD HVGSLSEKLA AVVNNLNTGQ VLHVVQALYP FSSSNDEELN FEKGDVMDVI EKPENDPEWW KCRKINGMVG LVPKNYVTVM QNNP, corresponding to amino acids 1-254 of Human Nck produced in E. coli.
MAEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKS WWR VRNSMNKTGF VPSNYVERKN SARKASIVKN LKD TLGIGKV KRKPSVPDSA SPADDSFVDP GERLYDLNMP AYVKFNYMAE REDELSLIKG TKVIVMEKCS DGWWRGS YNG QVGWFPSNYV TEEGDSPLGD HVGSLSEKLA AVV NNLNTGQ VLHVVQALYP FSSSNDEELN FEKGDVMDVI EKPENDPEWW KCRKINGMVG LVPKNYVTVM QNNP
Positive control
Non stimulated A431 kidney cell lysate or NIH3T3 cells.
Properties
Form
Liquid
Storage instructions
Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.1M Tris glycine, pH 7.0
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Signal transduction >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 42 kDa.
Target
Function
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.
Sequence similarities
Contains 1 SH2 domain.
Contains 3 SH3 domains.
Post-translational
modifications
Phosphorylated on Ser and Tyr residues.
Cellular localization
Cytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
Target information above from: UniProt accessionP16333
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cytoplasmic protein NCK1 antibody
- Melanoma Nck protein antibody
- MGC12668 antibody
see all
Database links
- Entrez Gene: 4690 Human
- Entrez Gene: 17973 Mouse
- Entrez Gene: 300955 Rat
- Omim: 600508 Human
- SwissProt: P16333 Human
- SwissProt: Q99M51 Mouse
- SwissProt: B2RZ33 Rat
- Unigene: 477693 Human
References for Anti-Nck antibody (ab14587)
ab14587 has not yet been referenced specifically in any publications.
Publishing research using ab14587? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"