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SDS-PAGE - Nck protein (ab40622)
(enlarge)
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Product Name
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Nck protein (His tag)
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Product type
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Proteins and Peptides
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Peptide
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Recombinant Nck fusion protein.
Fusion: His-tag at N-terminus.
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Tested applications
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MS, SDS-PAGE
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Application notes
(see key)
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Recommended dilutions
MS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Signal transduction >> Other
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
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Relevance
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Nck is a highly conserved, oncogenic protein. It is a common target for the action of different surface receptors, encoding one SH2 and three SH3 domains, the Src homology motifs found in nonreceptor tyrosine kinases, Ras GTPase activating protein, phosphatidylinositol 3 kinase, and phospholipase Cg. Nck is widely expressed in various tissues and in cell lines from human, murine, and rat origins. Nck is phosphorylated on tyrosine, serine, and threonine residues in response to stimulation of EGF and PDGF in A431 and NIH 3T3 cells respectively. Like other SH2 containing proteins, Nck is associated with tyrosine autophosphorylated EGF or PDGF receptors via its SH2 domain. Overexpression of Nck leads to transformation of NIH 3T3 cells.
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: 0.002% Sodium Azide
Constituents: 3mM Sodium chloride, 2.5mM Imidazole, 10mM Tris, pH 8.0 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.518 mg/ml
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Storage instructions
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Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
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Expression system
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E. coli
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Nck protein (His tag) is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Nck protein (His tag) images, references, products related to ab40622 and other tools.
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Nck protein (His tag) images:
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 SDS-PAGE - Nck protein (ab40622)
Analysis of Nck Recombinant Protein (1µg). 4-20% SDS gradient gel. Coomassie blue staining.
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Search PubMed (MEDLINE) for references to Nck
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Nck protein (His tag) - more information
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