Recombinant Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11521(B)] to Ndufs1
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Ndufs1 antibody [EPR11521(B)]
See all Ndufs1 primary antibodies -
Description
Rabbit monoclonal [EPR11521(B)] to Ndufs1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Raji, HepG2, HeLa and Jurkat cell lysates. Human cardiac muscle and Human liver tissue. HepG2 and permeabilized Jurkat cells.
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General notes
Ndufs1 is a protein encoded by the NDUFS1 gene. The NDUFS1 protein is the largest subunit of complex 1 which is located on the inner mitochondrial membrane. This complex is critical to the oxidative phosphorylation which occurs within the mitochondria.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11521(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/100 - 1/250.
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WB | (2) |
1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 79 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
1/10 - 1/100.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/100 - 1/250. |
WB
1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 79 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
Target
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Function
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. -
Involvement in disease
Defects in NDUFS1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
Sequence similarities
Belongs to the complex I 75 kDa subunit family.
Contains 1 2Fe-2S ferredoxin-type domain. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4719 Human
- Entrez Gene: 227197 Mouse
- Entrez Gene: 301458 Rat
- Omim: 157655 Human
- SwissProt: P28331 Human
- SwissProt: Q91VD9 Mouse
- SwissProt: Q66HF1 Rat
- Unigene: 471207 Human
see all -
Alternative names
- CI-75kD antibody
- Complex I 75Kd antibody
- Complex I, mitochondrial respiratory chain, 75 kD subunit antibody
see all
Images
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All lanes : Anti-Ndufs1 antibody [EPR11521(B)] (ab169540) at 1/10000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 79 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 7 secondsBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-Ndufs1 antibody [EPR11521(B)] (ab169540) at 1/10000 dilution
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) without mitochondria fraction cell lysate
Lane 2 : HepG2 mitochondria fraction cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 79 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking and diluting buffer: 5% NFDM/TBST
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Immunofluorescent analysis of 80% Methanol-fixed, 0.1% Triton X-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labelling Ndufs1 with ab169540 at 1/50 dilution (2.6 ug/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).
Confocal image showing cytoplasmic staining in Raji cell line.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).
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Ndufs1 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate with AB169540 at 1/20 dilution (0.6ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using AB169540 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 2: AB169540 IP in Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of AB169540 in Raji whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 7 seconds
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Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling Ndufs1 with ab169540 at 1/100 (1.29 ug/ml) followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on human kidney. The section was incubated with ab169540 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution1) for 20 min.
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Immunohistochemical analysis of paraffin-embedded rat kidney tissue labelling Ndufs1 with ab169540 at 1/100 (1.29 ug/ml) followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on rat kidney. The section was incubated with ab169540 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution1) for 20 min.
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling Ndufs1 with ab169540 at 1/100 (1.29 ug/ml) followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on mouse kidney. The section was incubated with ab169540 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution1) for 20 min.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labelling Ndufs1 with ab169540 at 1/20 dilution (1 ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab169540 has been referenced in 23 publications.
- Ledahawsky LM et al. The mitochondrial protein Sideroflexin 3 (SFXN3) influences neurodegeneration pathways in vivo. FEBS J 289:3894-3914 (2022). PubMed: 35092170
- Contreras L et al. Broad Transcriptomic Impact of Sorafenib and Its Relation to the Antitumoral Properties in Liver Cancer Cells. Cancers (Basel) 14:N/A (2022). PubMed: 35267509
- Wu Y et al. Dietary methionine deficiency stunts growth and increases fat deposition via suppression of fatty acids transportation and hepatic catabolism in Pekin ducks. J Anim Sci Biotechnol 13:61 (2022). PubMed: 35581591
- Jin X et al. Glycyrrhetinic acid restricts mitochondrial energy metabolism by targeting SHMT2. iScience 25:104349 (2022). PubMed: 35602963
- Tong WH et al. Hyperactivation of mTOR and AKT in a cardiac hypertrophy animal model of Friedreich ataxia. Heliyon 8:e10371 (2022). PubMed: 36061025