Anti-Ndufs1 antibody (ab185733)
Key features and details
- Rabbit polyclonal to Ndufs1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Ndufs1 antibody
See all Ndufs1 primary antibodies -
Description
Rabbit polyclonal to Ndufs1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Chimpanzee, Cynomolgus monkey, Gorilla, Orangutan -
Immunogen
Recombinant fragment corresponding to Human Ndufs1 aa 50-300.
Database link: P28331 -
Positive control
- HeLa, Raji, H460, PC3, 293T, HepG2, Mouse kidney, Mouse heart and Mouse intestine tissue extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 79 kDa.
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IHC-P |
1/50 - 1/200.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 79 kDa. |
IHC-P
1/50 - 1/200. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. -
Involvement in disease
Defects in NDUFS1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
Sequence similarities
Belongs to the complex I 75 kDa subunit family.
Contains 1 2Fe-2S ferredoxin-type domain. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 288380 Cow
- Entrez Gene: 4719 Human
- Entrez Gene: 227197 Mouse
- Entrez Gene: 301458 Rat
- Omim: 157655 Human
- SwissProt: Q0MQG2 Chimpanzee
- SwissProt: P15690 Cow
- SwissProt: Q4R6K9 Cynomolgus monkey
see all -
Alternative names
- CI-75kD antibody
- Complex I 75Kd antibody
- Complex I, mitochondrial respiratory chain, 75 kD subunit antibody
see all
Images
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All lanes : Anti-Ndufs1 antibody (ab185733) at 1/500 dilution
Lane 1 : HeLa cell line extract
Lane 2 : Raji cell line extract
Lane 3 : H460 cell line extract
Lane 4 : PC3 cell line extract
Lane 5 : 293T cell line extract
Lane 6 : HepG2 cell line extract
Lane 7 : Mouse kidney tissue extract
Lane 8 : Mouse heart tissue extract
Lane 9 : Mouse intestine tissue extract
Predicted band size: 79 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue labelling Ndufs1 with ab185733 at 1/200. Magnification: 400x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Ndufs1 with ab185733 at 1/200. Magnification: 400x.
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Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab185733. Blue DAPI for nuclear staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab185733 has been referenced in 1 publication.
- Maio N et al. Disruption of cellular iron homeostasis by IREB2 missense variants causes severe neurodevelopmental delay, dystonia and seizures. Brain Commun 4:fcac102 (2022). PubMed: 35602653