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Mouse IgG1 [CT6] - Isotype control (ab81216)

Q&A (3)Specific References (2)

Overview

  • Product nameMouse IgG1 [CT6] - Isotype controlSee all Mouse isotype controls ...
  • SpecificityThe antibody reacts with a Guinea pig lymphocyte subset probably analog to human CD8 (cytotoxic/suppressor) subset. Although this monoclonal originally was developed for the detection of a Guinea pig lymphocyte subset, it also can be used as a negative control for the mouse IgG1 subclass. It is sufficient for 100 tests. The antibody does not react with the 170-180 kD glycoprotein which is strongly correlated with the degree of Multi Drug Resistance. The antibody is sufficient for 100 tests.
  • Tested applicationsWB, Flow Cyt, IHC-P more details
  • Species reactivity
    Reacts with: Guinea pig
  • Immunogen

    The details of the immunogen for this antibody are not available.

    • Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
  • Concentration information loading...
  • PurityIgG fraction
  • Clonality Monoclonal
  • Clone numberCT6
  • IsotypeIgG1
  • Research Areas
    • Alternative names
        Mouse Isotype Control

    Applications

    Our Abpromise guarantee covers the use of ab81216 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent concentration.
    Flow Cyt Flow Cyt: Use at an assay dependent concentration.
    IHC-P IHC-P: 1/20.

    References for Mouse IgG1 [CT6] - Isotype control (ab81216)

    This product has been referenced in:
    • Scheper RJ  et al. Monoclonal antibody JSB-1 detects a highly conserved epitope on the P-glycoprotein associated with multi-drug-resistance. Int J Cancer 42:389-94 (1988). Read more (PubMed: 2458323) »
    • Tan BT  et al. Production of monoclonal antibodies defining guinea pig T-cell surface markers and a strain 13 Ia-like antigen: the value of immunohistological screening. Hybridoma 4:115-24 (1985). Guinea pig . Read more (PubMed: 3891587) »

    See all 2 Publications for this product

    Publishing research using ab81216 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab81216

    Displaying 1 - 3 of 3 results for Q&A

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    Votes: Highest First

    I purchased Anti-Negative control for mouse IgG1 Ab (ab81216) to assess the effect of other blocking ab on tumor cell growth.
    But, what I found was cells treated with IgG1 ab also showed retarded growth similar with blocking ab.
    I am wondering i...

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    Thank you for contacting us.

    We have not heard other reports of this, but it is possible the azide in the antibody, which is present at 0.1% in the stock solution, does have an effect on the tumor growth. Confirming this would require an experi...

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    Please send us CofC for these two items.

    Thank you for your reply.

    I have attached the certificate of compliance for ab290 and ab81216, with the lot numbers specified.

    I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact u...

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    Please send me CofA for the followings:

    Cat# AB290 Lot# GR7657-1

    Cat# AB81216 Lot# GR98155-1

    Thanks

    Thank you for your enquiry.

    Some of our antibodies are produced in-house and others are sourced from suppliers or academic collaborators whose products we believe will be of interest to our customers. All these products are guaranteed by our Ab...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"