Anti-Nesprin3 antibody [Nsp3] (ab123031)
Key features and details
- Mouse monoclonal [Nsp3] to Nesprin3
- Suitable for: ICC/IF
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-Nesprin3 antibody [Nsp3]
See all Nesprin3 primary antibodies -
Description
Mouse monoclonal [Nsp3] to Nesprin3 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse
Does not react with: Human, Zebrafish -
Immunogen
Recombinant fragment corresponding to Mouse Nesprin3. Fusion protein encoding the seventh spectrin repeat of Nesprin3 and a proprietary protein.
Database link: Q4FZC9 -
Positive control
- Mouse embryonic fibroblasts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
Nsp3 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab123031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. -
Sequence similarities
Belongs to the nesprin family.
Contains 1 KASH domain.
Contains 2 spectrin repeats. -
Domain
The KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains. -
Cellular localization
Nucleus outer membrane. Located at the rough reticulum endoplasmic (RER) in some cells. - Information by UniProt
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Database links
- Entrez Gene: 212073 Mouse
- SwissProt: Q4FZC9 Mouse
- Unigene: 212688 Mouse
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Alternative names
- C14orf49 antibody
- Chromosome 14 open reading frame 49 antibody
- FLJ25605 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab123031 has been referenced in 3 publications.
- Pieuchot L et al. Curvotaxis directs cell migration through cell-scale curvature landscapes. Nat Commun 9:3995 (2018). PubMed: 30266986
- Alam SG et al. The mammalian LINC complex regulates genome transcriptional responses to substrate rigidity. Sci Rep 6:38063 (2016). ICC/IF . PubMed: 27905489
- Gutiérrez-Fernández A et al. Loss of MT1-MMP causes cell senescence and nuclear defects which can be reversed by retinoic acid. EMBO J 34:1875-88 (2015). PubMed: 25991604